04-746
Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325, rabbit monoclonal
culture supernatant, clone JY325, Upstate®
别名:
H3T3P, Histone H3 (phospho T3)
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
JY325, monoclonal
Species reactivity:
human
Application:
DB, WB, inhibition assay, multiplexing
Citations:
8
biological source
rabbit
antibody form
culture supernatant
antibody product type
primary antibodies
clone
JY325, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
dot blot: suitable, inhibition assay: suitable (peptide), multiplexing: suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pThr37)
Quality Level
Gene Information
human ... H3C1(8350)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
17 kDa
Immunogen
Peptide containing the sequence [RTtrimKQ] in which lysine 4 is trimethylated on human histone H3
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-phospho-Histone H3 (Thr3) Antibody, clone JY325 (Rabbit Monoclonal Antibody) validated in WB, DB, Mplex, PIA to detect phospho-Histone H3 (Thr3) also known as H3T3P, Histone H3 (phospho T3).
WB, ChIP, BD, PIA
Biochem/physiol Actions
Histone H3 phosphorylated at Threonine 3; cross-reactivity with phospho-histone H3 Thr22 detected in some applications
Physical form
Cultured supernantant in 0.05% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid
Acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid
routinely evaluated by immunoblot on acid extracted proteins from mitotic HeLa cells (Catalog #17-306) treated with colcemid, but not unmodified recombinant histone H3
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
D Huertas et al.
Oncogene, 31(11), 1408-1418 (2011-08-02)
The approval of histone deacetylase inhibitors for treatment of lymphoma subtypes has positioned histone modifications as potential targets for the development of new classes of anticancer drugs. Histones also undergo phosphorylation events, and Haspin is a protein kinase the only
Svetlana M Medvedeva et al.
Molecules (Basel, Switzerland), 27(13) (2022-07-10)
This study represents the design and synthesis of a new set of hybrid and chimeric derivatives of 4,5-dihydro-4,4-dimethyl-1H-[1,2]dithiolo[3,4-c]quinoline-1-thiones, the structure of which the tricyclic fragment linearly bound or/and condensed with another heterocyclic fragment. Using the PASS Online software, among the
Jing Wang et al.
The Plant journal : for cell and molecular biology, 91(2), 199-207 (2017-04-04)
1RS.1BL translocations are centric translocations formed by misdivision and have been used extensively in wheat breeding. However, the role that the centromere plays in the formation of 1RS.1BL translocations is still unclear. Fluorescence in situ hybridization (FISH) was applied to detect
Gabrielle R Budziszewski et al.
Nucleic acids research, 50(8), 4355-4371 (2022-04-08)
A key role of chromatin kinases is to phosphorylate histone tails during mitosis to spatiotemporally regulate cell division. Vaccinia-related kinase 1 (VRK1) is a serine-threonine kinase that phosphorylates histone H3 threonine 3 (H3T3) along with other chromatin-based targets. While structural
Franziska Böttger et al.
Cell reports, 27(11), 3345-3358 (2019-06-13)
Small-cell lung cancer is the most aggressive type of lung cancer, characterized by a remarkable response to chemotherapy followed by development of resistance. Here, we describe SCLC subtypes in Mycl- and Nfib-driven GEMM that include CDH1-high peripheral primary tumor lesions
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