Akt is the major known effector of the PI3 Kinase pathway. Generation of PIP3 results in the activation of PDK1, which phosphorylates Akt on Thr308, and another kinase complex, thought to be the mTORC2 complex (or possibly an intermediate kinase linking the two) that phosphorylates Akt on Ser473. These phosphorylations additively activate Akt Ser/Thr kinase activity, and the use of phosphorylation state-specific antibodies directed against either of these sites can imply Akt activation. Activation of Akt can be measured directly by immunoprecipitation followed by phosphorylation of a known substrate with radiolabeled ATP. Akt has been shown to phosphorylate over 70 substrates including TSC1&2 in the mTOR pathway, Bad on Ser136, GSK3 (which is inactivated by this phosphorylation), and the FoxO family of transcription factors (PRAS40, AS160).

~60 kDa

Epitope: a.a. 455-469

KLH-conjugated, synthetic peptide (CRYDSLGSLELDQRTH) corresponding to amino acids 455-469 of human Akt2/PKBβ, with an N-terminal cysteine added for conjugation purposes.

Anti-Akt2/PKBβ Antibody, clone AW114 detects level of Akt2/PKBβ & has been published & validated for use in WB & IP.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

Predicted to cross-react with mouse.

Recognizes Akt2/PKBβ, Mr ~60 kDa.

Cultured supernantant containing 0.05% sodium azide.

Format: Purified

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
L6 cell lysate

Western Blot Analysis: A 1:500 dilution of this antibody detected Akt2/PKBβ in HepG2 lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-406

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