04-825
Anti-PDGFRβ Antibody, clone 4A56, rabbit monoclonal
culture supernatant, clone 4A56, Upstate®
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
4A56, monoclonal
Application:
IP, WB
Citations:
2
biological source
rabbit
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
4A56, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PDGFRB(5159)
General description
190kDa
Immunogen
KLH-conjugated, synthetic peptide corresponding to amino acids 710-718 of human PDGF Receptor β. An N-terminus cysteine was added to facilitate conjugation. This sequence corresponds to amino acids 709-717 of mouse PDGF Receptor β.
Application
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
This Anti-PDGFRβ Antibody, clone 4A56 is validated for use in IP, WB for the detection of PDGFRβ.
Westerm Blot Analysis:
A 1:2,000 dilution of this lot detected PDGFRβ in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells.
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated PDGFRβ from 500 μg of 3T3/A31 RIPA lysate.
A 1:2,000 dilution of this lot detected PDGFRβ in RIPA lysates from mouse NIH/3T3, 3T3/A31, and human HFF cells.
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated PDGFRβ from 500 μg of 3T3/A31 RIPA lysate.
Biochem/physiol Actions
PDGFRβ, does not cross-react with EGF receptor
Physical form
Cultured supernantant in 0.05% sodium azide
Preparation Note
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Control
RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
routinely evaluated by immunoblot on RIPA lysates from mouse NIH/3T3, 3T3/A31, or human HFF cells
Other Notes
Replaces: 05-825
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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