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04-841

Sigma-Aldrich

Anti-ROKα/ROCK-II Antibody, clone A9W4, rabbit monoclonal

culture supernatant, clone A9W4, from rabbit

别名:

RhoA-binding kinase 2, Rho-associated, coiled-coil containing protein kinase 2, Rho-associated, coiled-coil-containing protein kinase II, p150 ROK-alpha, p164 ROCK-2, ROCK-II, ROKalpha, Rock2

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1 EA
¥11,367.38

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预计发货时间2025年9月10日详情


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1 EA
¥11,367.38

About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

¥11,367.38


预计发货时间2025年9月10日详情


获取大包装报价

生物来源

rabbit

质量水平

偶联物

unconjugated

抗体形式

culture supernatant

抗体产品类型

primary antibodies

克隆

A9W4, monoclonal

种属反应性

rat, human, mouse, bovine

技术

immunoprecipitation (IP): suitable
western blot: suitable

NCBI登记号

UniProt登记号

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此商品
L4277L852562285
biological source

Aspergillus sp. (Aspergillus oryzae)

biological source

-

biological source

fungus (Candida rugosa)

biological source

-

technique(s)

cell based assay: suitable

technique(s)

-

technique(s)

-

technique(s)

-

specific activity

≥100,000 U/g

specific activity

≥20,000 U/g

specific activity

≥40,000 units/mg protein

specific activity

~50 U/mg

form

solution

form

lyophilized

form

lyophilized powder

form

powder

UniProt accession no.

I8ABR3, I8ADK3

UniProt accession no.

-

UniProt accession no.

-

UniProt accession no.

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

一般描述

Rho-Associated Kinase (Rho-Kinase or ROK) has been classified into two isoforms: ROKα/ROCK-II and ROKβ/ROCK-I. These large cellular proteins (150-160 kDa), have been reported to be involved in the formation of stress fibers, focal adhesions and cardiac hypertonic responses. Current research suggests that ROK of smooth muscle may be involved in the contractile process via phosphorylation of MYPT1 and Myosin.

特异性

Predicted to cross-react with mouse and bovine based on sequence homology.
Recognizes ROKα/ROCK-II, Mr 150 kDa. Does not recognize ROKβ/ROCK-I. A non-specific band may also be detected, Mr 80 kDa.

免疫原

6His-tagged fusion protein corresponding to residues 2-543 of rat ROKα/ROCK-II.

应用

Detect ROKα/ROCK-II using this Anti-ROKα/ROCK-II Antibody, clone A9W4 validated for use in IP & WB.
Immunoprecipitation:
4 μL of a previous lot immunoprecipitated ROKα/ROCK-II from 500 μg of PC-12 lysate.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

质量

Routinely evaluated by Western BLot on RIPA lysates from PC-12 cells.

Western Blot Analysis: A 1:500-1:5000 dilution of this antibody detected ROKα/ROCK-II in RIPA lysates from PC-12 cells.

目标描述

150 kDa

联系

Replaces: 05-841

外形

Cultured supernatant containing 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
RIPA lysates from PC-12 cells

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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[1, 3] Oxazolo [3, 2-b][1, 2, 4] triazoles: a versatile synthesis of a novel heterocycle
Ball Catherine, et al.
Tetrahedron Letters, 51(30), 3907-3909 (2010)
Synthesis of 2, 4-and 2, 4, 5-substituted oxazoles via a silver triflate mediated cyclization
Bailey JL and Sudini RR
Tetrahedron Letters, 55(27) (2014)
Chai Hoon Soh et al.
Journal of combinatorial chemistry, 10(1), 118-122 (2007-12-28)
A microwave-assisted protocol was developed for the construction of di- and monosubstituted 2-aminoimidazoles. The two-step reaction involves the synthesis of N-(1H-imidazol-2-yl)acetamides from readily available alpha-haloketones and N-acetylguanidine, followed by deacetylation. Significant rate enhancement was observed for both steps of the

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