Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T, rabbit monoclonal

Calmodulin Binding Protein (CBP) epitope tags are found in several E. coli expression vectors. Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+ binding protein, acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase. Calmodulin is the principal intracellular calcium receptor. It is an acidic protein of 148 residues, and upon binding Ca2+, it in turn binds to short peptide sequences of many target proteins. Such interaction is thought to induce a conformational change on the target, resulting in its activation. Targets include the constitutively expressed nitric oxide synthases, the CaM kinases, and PP2B/Calcineurin.

The molecular weight is related to the tagged protein.

Epitope: CBP epitope

KLH-conjugated, synthetic peptide corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

This Anti-Calmodulin Binding Protein Epitope Tag Antibody, clone C16T is validated for use in WB for the detection of Calmodulin Binding Protein Epitope Tag.

Recombinant proteins containing the calmodulin binding protein epitope tag.

Cultured supernatant with 0.05% sodium azide.

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Samples containing the calmodulin binding protein epitope tag.

Evaluated by Western Blot on lysates from transformed E. coli.
Western Blot Analysis: 1:2,000 dilution of this antibody detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.

Replaces: 05-932

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