05-1087
Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2
clone 24.6.2, from mouse
别名:
insulin receptor substrate 1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
24.6.2, monoclonal
Species reactivity:
bovine, pig, mouse, monkey, canine, rat, human
Application:
ICC, IF, IP, WB
Citations:
13
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
24.6.2, monoclonal
species reactivity
bovine, pig, mouse, monkey, canine, rat, human
technique(s)
immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer307 )
Gene Information
bovine ... Irs1(538598)
dog ... Irs1(486148)
human ... IRS1(3667)
mouse ... Irs1(16367)
pig ... Irs1(100512686)
rat ... Irs1(25467)
rhesus monkey ... Irs1(707870)
General description
IRS1 (Insulin Receptor Substrate 1) transmits insulin signals via metabolic and mitogenic pathways. IRS1 is heavily phosphorylated on both serine and tyrosine residues. These phosphorylated tyrosines enable IRS to act as a docking protein that binds SH2 domains of such proteins as PI3 Kinase (phosphatidylinositol 3-kinase) and GRB2, resulting in activation. Over expression and phosphorylation of serine is associated with insulin resistance and breast cancer. Some of the more notable phosphorylation sites are Ser302 that is phosphorylated following insulin stimulation. Ser307, phosphorylated by JNK and IKK, is a key regulatory site that appears to disrupt the IRS1/IR interaction and inhibits insulin-mediated activation of the PI3 kinase and MAPK pathways, and Ser636/639 that is known to be phosphorylated by p70S6K downstream of mTOR and acts as a negative feedback loop.
~185 kDa
Immunogen
Synthetic peptide corresponding to amino acids surrounding phosphorylated Ser307 of mouse IRS1.
Application
This Anti-phospho-IRS1 (Ser307 mouse/ Ser312 human) Antibody, clone 24.6.2 is validated for use in WB, IP, IC, IF for the detection of phospho-IRS1 (Ser307 mouse/ Ser312 human).
Biochem/physiol Actions
Predicted to cross-react with many other species based on 100% sequence homology with immunogen.
This antibody recognizes IRS1 phosphorylated at Ser307 (mouse) and Ser312 (human).
Physical form
Format: Purified
Analysis Note
Evaluated by western blot on IR/IRS1 transfected CHO +/- Insulin lysate.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect IRS1 in IRS/IR transfected CHO -/+ Calyculin A/ Okadaic Acid-treated cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 07-247
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable