产品名称
Anti-Giα2 Antibody, clone L5, clone L5, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
L5, monoclonal
species reactivity
guinea pig (43%), rat (69%), bovine (100%), human (62%), mouse (69%)
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GNAI2(2771)
Analysis Note
Control
Huvec cell lysate
Huvec cell lysate
Routinely evaluated by Western Blot on Huvec lysates.
Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.
Western Blot Analysis: 1:500 dilution of this lot detected G I ALPHA 2 on 10 μg of Huvec lysates.
Application
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer
Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of G-Protein Monoclonal Antibody: Testicular Cancer
Western Blot: A previous lot of this antibody was used at 1:500 - 1:1000, with detection by 125l-labeled anti-IgG. Labels a single protein band of 39 - 42 kDa in bovine and rat membranes
Research Category
Signaling
Signaling
Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling
GPCR, cAMP/cGMP & Calcium Signaling
This Anti-Giα2 Antibody, clone L5 is validated for use in WB, IH(P) for the detection of Giα2.
Biochem/physiol Actions
Specificity confirmed by Western blot versus bovine Gαt, rat Goα, rat Giα-1, Giα-2, rat Gia-3, bovine Gsα, and murine GoαA and GoαB.
This antibody recognizes Giα-2.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Labels a single protein band of 39-42 kDa in bovine and rat membranes.
Immunogen
Recombinant Giα-2, purified by the method of Linder (J. Biol. Chem. [1990] 265, 8243).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3077
Physical form
Format: Purified
Purified
Purified mouse monoclonal IgG2b, in 0.02 M phosphate buffer, 0.25 M sodium chloride, pH 7.6, with 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C for 1 year from date of receipt.
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Saori Yano et al.
Chemical senses, 40(9), 641-648 (2015-09-18)
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Jennifer M Lin et al.
Developmental biology, 441(1), 67-82 (2018-06-22)
The identity of individual neuronal cell types is defined and maintained by the expression of specific combinations of transcriptional regulators that control cell type-specific genetic programs. The epithelium of the vomeronasal organ of mice contains two major types of vomeronasal
Ankana S Naik et al.
Development (Cambridge, England), 147(8) (2020-04-29)
The vomeronasal organ (VNO) contains two main types of vomeronasal sensory neurons (VSNs) that express distinct vomeronasal receptor (VR) genes and localize to specific regions of the neuroepithelium. Morphogenic signals are crucial in defining neuronal identity and network formation; however
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