biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
18E4, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SYNCRIP(10492)
General description
克隆18E4在总HeLa细胞裂解液的蛋白质印迹上可识别~80、~70、~60和~55 kDa的4条条带,hnRNP Q具有~70– 55 kDa的3个亚型,其中~80 kDa的活性条带被鉴定为hnRNP R,~80 kDa时在SDS–PAGE上迁移。(Mourelatos., Z., et al., EMBO J., 20, 5443-5452 (2001)。)
异质核核糖核蛋白Q通常称为hnRNP Q,是由单个基因的选择性剪接产生的三种不同亚型。这种RNA结合蛋白与运动神经元(SMN)蛋白存活的mRNA前体剪接控制有关。SMN的功能丧失突变与脊髓性肌萎缩症(SMA)有关,SMA是一种常见的神经退行性疾病。研究表明hnRNP Q蛋白可与SMN相互作用,这种相互作用是有效的体外pre-mRNA剪接所必需的。目前的研究表明hnRNP Q是SMN的剪接调制器。
Immunogen
对应于人hnRNP Q的重组蛋白。
表位:未知
Application
使用在WB、ICC、IP中验证的抗-hnRNP Q抗体,克隆18E4(小鼠单克隆抗体)检测hnRNP Q,也称Q样异质核糖核蛋白。
免疫细胞化学
由独立研究人员使用代表性批次进行报道。
免疫沉淀
由独立研究人员使用代表性批次进行报道。
由独立研究人员使用代表性批次进行报道。
免疫沉淀
由独立研究人员使用代表性批次进行报道。
研究子类别
RNA代谢&结合蛋白
RNA代谢&结合蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
该抗体可识别hnRNP Q。
Physical form
形式:纯化
纯化的小鼠单克隆IgGκ,溶于含有0.1 M Tris-甘氨酸(pH 7.4,150 mM NaCl)和0.05%叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起,在2-8°C条件下可稳定保存1年。
Analysis Note
对照
HeLa细胞裂解液
HeLa细胞裂解液
通过蛋白质印迹法在HeLa细胞裂解液中进行了评估。
蛋白质印迹分析:1 µg/ml该抗体可在10 µg HeLa细胞裂解液中检测到hnRNP。
蛋白质印迹分析:1 µg/ml该抗体可在10 µg HeLa细胞裂解液中检测到hnRNP。
Other Notes
浓度:请参考批次特异性浓缩物的检验报告。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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