05-1579
Anti-Axin-1 Antibody, clone A5
clone A5, from mouse
别名:
Axis inhibition protein 1, axin 1, axis inhibitor 1, fused, mouse, homolog of
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
A5, monoclonal
Application:
ICC, IP, WB
Citations:
6
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
A5, monoclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AXIN1(8312)
mouse ... Axin1(12005)
General description
Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.
~ 97 kDa
Immunogen
GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.
Application
Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.
Biochem/physiol Actions
This antibody recognizes the N-terminus of Axin-1.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable