05-1579
Anti-Axin-1 Antibody, clone A5
clone A5, from mouse
别名:
Axis inhibition protein 1, axin 1, axis inhibitor 1, fused, mouse, homolog of
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
A5, monoclonal
Application:
ICC, IP, WB
Citations:
6
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
A5, monoclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... AXIN1(8312)
mouse ... Axin1(12005)
General description
Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.
~ 97 kDa
Immunogen
GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.
Application
Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.
Biochem/physiol Actions
This antibody recognizes the N-terminus of Axin-1.
Physical form
Format: Purified
Analysis Note
Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Zbed3 contributes to malignant phenotype of lung cancer via regulating β-catenin and P120-catenin 1.
Chuifeng Fan et al.
Molecular carcinogenesis, 54 Suppl 1, E138-E147 (2014-09-30)
Our previous studies indicate that abnormal expression of several Wnt signaling molecules including Axin, Dvl and β-catenin are involved in proliferation, invasion and metastasis of lung cancer. Zbed3 was found to inhibit function of Axin-GSK3β complex and thus lead to
Phosphatidylinositol 3-kinase signaling does not activate the wnt cascade.
Ng, SS; Mahmoudi, T; Danenberg, E; Bejaoui, I; de Lau, W; Korswagen, HC; Schutte, M; Clevers, H
The Journal of Biological Chemistry null
S Monteagudo et al.
Osteoarthritis and cartilage, 30(5), 724-734 (2022-03-02)
To investigate how ANP32A, previously linked to the antioxidant response, regulates Wnt signaling as unraveled by transcriptome analysis of Anp32a-deficient mouse articular cartilage, and its implications for osteoarthritis (OA) and diseases beyond the joint. Anp32a knockdown chondrogenic ATDC5 cells were
Rong Xie et al.
eLife, 11 (2022-12-22)
Axin1 is a key regulator of canonical Wnt signaling pathway. Roles of Axin1 in skeletal development and in disease occurrence have not been fully defined. Here, we report that Axin1 is essential for lower limb development. Specific deletion of Axin1
Eunsun Kim et al.
Nature communications, 10(1), 1909-1909 (2019-04-25)
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related deaths worldwide. β-catenin is widely thought to be a major oncogene in HCC based on the frequency of mutations associated with aberrant Wnt signaling in HCC patients. Challenging this model
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