产品名称
抗-PCNA抗体，克隆PC10, clone PC10, Upstate®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
culture supernatant
antibody product type
primary antibodies
clone
PC10, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... PCNA(5111)
Analysis Note
对照
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。
已通过免疫印迹对来自人A431细胞的RIPA 裂解液或小鼠3T3/A31细胞裂解液进行了常规评估
Application
用抗PCNA抗体（克隆PC10，小鼠单克隆抗体）检测PCNA，该抗体已证明可在WB、ICC&IHC中起作用。
研究子类别
细胞周期，DNA 复制&修复
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
PCNA
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
36kDa
增殖细胞核抗原（PCNA），即细胞周期素或称聚合酶δ辅助蛋白，其表达在G1 晚期DNA合成以前上升，在S期达到最大，在G2和M期下降。PCNA/细胞周期素可作为DNA聚合酶δ的辅助蛋白，在细胞增殖的引发中起到重要作用。抗PCNA 抗体在蛋白质印迹中与分子量为36 kDa的PCNA反应。
Immunogen
重组大鼠PCNA
Physical form
形式：纯化
浓缩的培养上清液
蛋白A纯化的小鼠免疫球蛋白，溶于含1 mg/mL BSA和10mM叠氮化的PBS中。
Preparation Note
在-20°C下可保存2年
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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