产品名称
Anti-phospho-Raf-1 (Ser338) Antibody, Upstate®, from mouse
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity
mouse
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer338)
Quality Level
Gene Information
human ... RAF1(5894)
Physical form
Format: Purified
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH 7.4.
Protein G Purified
Analysis Note
Control
Expressed in most tissue, lysates from 3T3/NIH cells stimulated with PDGF
Expressed in most tissue, lysates from 3T3/NIH cells stimulated with PDGF
routinely evaluated by immunoblot on RIPA lysates from 3T3/NIH cells stimulated with PDGF
Application
Anti-phospho-Raf-1 (Ser338) Antibody detects level of phospho-Raf-1 (Ser338) & has been published & validated for use in WB.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
Biochem/physiol Actions
Recognizes Raf-1 phosphorylated at Serine 338.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
74 kDa
Immunogen
Peptide corresponding to residues 333-345 of human Raf-1
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Regulation of mTORC1 signaling by pH.
Balgi, AD; Diering, GH; Donohue, E; Lam, KK; Fonseca, BD; Zimmerman, C; Numata, M; Roberge, M
Testing null
Culture of Drosophila primary cells dissociated from gastrula embryos and their use in RNAi screening.
Bai J, Sepp KJ, Perrimon N
Nature Protocols null
Raf-1 serine 338 phosphorylation plays a key role in adhesion-dependent activation of extracellular signal-regulated kinase by epidermal growth factor.
Edin, ML; Juliano, RL
Molecular and cellular biology null
Development of a highly reproducible three-dimensional organotypic model of the oral mucosa.
Anna Dongari-Bagtzoglou, Helena Kashleva
Nature Protocols null
Antonia K Roseweir et al.
Journal of the National Cancer Institute, 110(6), 616-627 (2018-06-20)
Aromatase inhibitors improve disease-free survival compared with tamoxifen in postmenopausal women with hormone receptor-positive breast cancer. The Tamoxifen and Exemestane Adjuvant Multinational (TEAM) trial compared exemestane monotherapy with sequential therapy of tamoxifen followed by exemestane. The trial failed to show
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