33kDa

Recombinant human cdk2

0.5-2μg/ml of this lot detected cdk2 in HeLa nuclear extract. 4μg of a previous lot immunoprecipitated cdk2 from 500μg of mouse 3T3 nuclear extract.

Detect Cdk2 with Anti-Cdk2 Antibody, clone AN4.3 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

cdk2, does not react with other cdks

Protein G Chromatography

0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide, and 30% glycerol.

Format: Purified

2 years at -20°C

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

routinely evaluated by immunoblot on HeLa nuclear extract

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