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Merck
CN

05-655

Anti-p21/WAF1/Cip1 Antibody, clone CP74

clone CP74, Upstate®, from mouse

别名:

Anti-CAP20, Anti-CIP1, Anti-MDA-6, Anti-P21, Anti-SDI1, Anti-WAF1, Anti-p21CIP1

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
CP74, monoclonal
Application:
immunohistochemistry
western blot
Species reactivity:
human
Citations:
32
Technique(s):
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
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产品名称

Anti-p21/WAF1/Cip1 Antibody, clone CP74, clone CP74, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

CP74, monoclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... CDKN1A(1026)

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
routinely evaluated by immunoblot on nuclear extract from HeLa cells

Application

Anti-p21/WAF1/Cip1 Antibody, clone CP74 is a high quality Mouse Monoclonal Antibody for the detection of p21/WAF1/Cip1 & has been validated in WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Biochem/physiol Actions

Cip1

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

21kDa

Immunogen

Recombinant full-length human Cip1 with an N-terminal histidine tag; epitope corresponds to amino acids 1-80 of full-length human Cip1

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein G Purified

Preparation Note

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1


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Yongye Huang et al.
Biomedical reports, 7(1), 36-40 (2017-07-08)
Wnt signaling is critical in embryonic development and post-embryonic tissue homeostasis. The aim of the present study was to evaluate the expression levels of canonical Wnt signaling genes in porcine somatic cell nuclear transfer (SCNT) embryos. Quantitative polymerase chain reaction
N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) triggers MSH2 and Cdt2 protein-dependent degradation of the cell cycle and mismatch repair (MMR) inhibitor protein p21Waf1/Cip1.
Jascur, T; Fotedar, R; Greene, S; Hotchkiss, E; Boland, CR
The Journal of Biological Chemistry null
STX140 and STX641 cause apoptosis via the intrinsic mitochondrial pathway and down-regulate survivin and XIAP expression in ovarian and prostate cancer cells.
Foster PA, Ho YT, Newman SP, Leese MP, Potter BV, Reed MJ, Purohit A
Anticancer research null
Z Y Yang et al.
Nature medicine, 1(10), 1052-1056 (1995-10-01)
The p21 gene encodes a cyclin-dependent kinase inhibitor that affects cell-cycle progression, but the potential of this gene product to serve as a tumour suppressor in vivo has not been established. In this report, we show that the growth of
Tobias Åkerström et al.
Scientific reports, 6, 19546-19546 (2016-01-28)
Primary aldosteronism (PA) is the most common cause of secondary hypertension with a prevalence of 5-10% in unreferred hypertensive patients. Aldosterone producing adenomas (APAs) constitute a large proportion of PA cases and represent a surgically correctable form of the disease.

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