Anti-Kaiso Antibody, clone 6F

97kDa

Recombinant N-terminal GST and His-tagged fusion protein corresponding to residues 103-584 of murine Kaiso

Detect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Kaiso

0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.

Format: Purified

Protein G Chromatography

2 years at -20°C

Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells.
Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999).
Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001).
Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

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