Anti-Myosin Heavy Chain Antibody, clone A4.1025

Myosin-1 (UniProt: P12882; also known as Myosin heavy chain 1, Myosin heavy chain 2x, MyHC-2x, Myosin heavy chain IIx/d, MyHC-IIx/d, Myosin heavy chain skeletal muscle adult 1) is encoded by the MYH1 gene (Gene ID: 4619) in human. Myosins are highly conserved, ubiquitously expressed proteins that form regular bipolar thick filaments that together with actin thin filaments constitute the fundamental contractile unit of skeletal and cardiac muscle. Along with actin they serve as motor molecules with ATPase activity that is essential for muscle contraction. Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2). Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular sub-fragments (S1) and 1 rod-shaped sub-fragment (S2). The globular head of myosin heavy chain contains the actin-activated ATPase. It has been reported that during rest, the myosin head does interact with the actin filament due to the presence of tropomyosin. However, when muscle contracts, tropomyosin shifts out of the way to allow the globular head to engage the actin filaments. Myosin heavy chain is a conventional myosin with a N-terminal SH3-like domain (aa 37-82), a myosin motor domain (aa 86-782), and an actin-binding domain (aa 761-775). It has a rod like tail sequence that is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils

Partially purified myosin from adult human skeletal muscle.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Myosin Heavy Chain in lysates from L6 cells and human skeletal muscle.

mmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Myosin Heavy Chain in human skeletal muscle tissue sections.

Immunohistochemistry Applications: A representative lot detected Myosin Heavy Chain in Immunohistochemistry applications

Western Blotting Analysis: A representative lot detected Myosin Heavy Chain in Western Blotting applications

Immunofluorescence Analysis: A representative lot detected Myosin Heavy Chain in Immunofluorescence applications

Immunocytochemistry Analysis: A representative lot detected Myosin Heavy Chain in Immunocytometry applications

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone A4.1025 is a mouse monoclonal antibody that detects Myosin Heavy Chain.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stable for 1 year at +2°C to +8°C from date of receipt.

Evaluated by Western Blotting in C2C12 cell lysate.

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.