05-806-AF555
Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor™ 555
clone 3H10, from mouse, ALEXA FLUOR™ 555
别名:
H3S10P, Histone H3 (phospho Ser10), H3 histone, family 3A, H3.3A, H3 histone, family 3B, H3.3B
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 555
Clone:
3H10, monoclonal
Application:
ICC
Citations:
-
biological source
mouse
conjugate
ALEXA FLUOR™ 555
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3H10, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10)
Quality Level
Gene Information
human ... H3C1(8350)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone H3 variants (H3.1, H3.2 and H3.3) have been implicated in the epigenetic memory of cellular state. Genome-wide patterns of H3 are dependent on amino acid sequence and change with cellular differentiation at developmentally regulated loci.
~17 kDa observed.
Immunogen
Epitope: H3 pSer10.
Application
Anti-phospho-Histone H3 (Ser10) Antibody, clone 3H10, Alexa Fluor™ 555 is an antibody against phospho-Histone H3 for use in Immunocytochemistry.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-Histone H3 (Ser10) in HepG2 cells.
The unconjugated antibody (Cat. No. 05-806) is shown to be suitable also for peptide inhibition assay (PIA), flow cytometry, immunocytochemistry, immunofluorescence, multiplex assay, Western blotting applications.
The unconjugated antibody (Cat. No. 05-806) is shown to be suitable also for peptide inhibition assay (PIA), flow cytometry, immunocytochemistry, immunofluorescence, multiplex assay, Western blotting applications.
Biochem/physiol Actions
Broad species cross-reactivity expected
Clone 3H10 recoginzes histone N-terminal peptide with Ser10 phosphorylation (pSer10) by bead-based peptide specificity assay. Clone 3H10 does not bind unphosphorylated peptides or peptides with only pThr11 or pSer28 modification.
Physical form
Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
Analysis Note
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H3 (Ser10) in HeLa cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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