05-938
Anti-ORC6 Antibody, clone 3A4
clone 3A4, Upstate®, from rat
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3A4, monoclonal
Application:
ChIP, WB
Citations:
4
biological source
rat
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3A4, monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ORC6(23594)
General description
30kDa A non specific protein was also detected at Mr 120kDa
Immunogen
Full-length human origin recognition complex 6 (ORC6). Clone 3A4.
Application
Anti-ORC6 Antibody, clone 3A4 is a high quality Rat Monoclonal Antibody for the detection of ORC6 & has been validated in ChIP & WB.
Biochem/physiol Actions
ORC6
Physical form
Format: Purified
Analysis Note
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Aparna Palakodeti et al.
Human molecular genetics, 19(1), 99-110 (2009-10-10)
Chromosomal common fragile sites (CFSs) are genetically unstable regions of the genome that are induced by conditions that impair DNA replication. In this report, we show that treatment with the DNA polymerase inhibitor, aphidicolin (APH), slows the replication rate throughout
Complex protein-DNA dynamics at the latent origin of DNA replication of Epstein-Barr virus.
Ritzi, Marion, et al.
Journal of Cell Science, 116, 3971-3984 (2003)
Isabelle Lucas et al.
EMBO reports, 8(8), 770-777 (2007-08-02)
Mapping origins of replication has been challenging in higher eukaryotes. We have developed a rapid, genome-wide method to map origins of replication in asynchronous human cells by combining the nascent strand abundance assay with a highly tiled microarray platform, and
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