Anti-LSD1/BHC110 Antibody

Histone demethylase that specifically demethylates ′Lys-4′ of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted ′Lys-4′ of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 ′Lys-4′ on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate ′Lys-9′ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

Recognizes LSD1, Mr 110 kDa.

GST fusion protein corresponding to residues 23-138 of human LSD1.

Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.

Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.

Immunoprecipitation:
Recommended.

Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)

Immunofluorescence:
Recommended.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

Routinely evaluated by western blot.

Western Blot Analysis:
1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.

110 kDa

Replaces: CBL770

Unpurified

Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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