生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
M110, monoclonal
种属反应性
rat, human, mouse
制造商/商品名称
Upstate®
技术
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG1
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... PRKCA(5578)
一般描述
80-82 kDa
免疫原
Recombinant protein of human PKCγ amino acids 499-697.
应用
Anti-PKC (α Antibody, β, γ), clone M110 detects level of PKC (α & has been published & validated for use in ELISA, IP, WB & IC.
生化/生理作用
Recognizes PKC α, β, γ. Does not cross react with other PKC isoforms.
外形
100 μL of protein A purified mouse monoclonal IgG1 in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
Format: Purified
制备说明
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
分析说明
Routinely evaluated by immunoblot.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Open biology, 7(1) (2017-01-20)
Tumour necrosis factor receptor 1 (TNFR1)-activated TNFα reverse signalling, in which membrane-integrated TNFα functions as a receptor for TNFR1, enhances axon growth from developing sympathetic neurons and plays a crucial role in establishing sympathetic innervation. Here, we have investigated the
Michael Rosenberg et al.
Molecular biology of the cell, 17(3), 1364-1374 (2006-01-06)
Nonmuscle myosin II is an important component of the cytoskeleton, playing a major role in cell motility and chemotaxis. We have previously demonstrated that, on stimulation with epidermal growth factor (EGF), nonmuscle myosin heavy chain II-B (NMHC-IIB) undergoes a transient
Amos M Sakwe et al.
The Journal of biological chemistry, 280(6), 4436-4441 (2004-12-02)
This study examined the mechanism of Ca2+ entry and the role of protein kinase C (PKC) in Ca2+ signaling induced by activation of the calcium sensing receptor (CaR) in HEK293 cells stably expressing the CaR. We demonstrate that influx of
Thomas T Su et al.
Nature immunology, 3(8), 780-786 (2002-07-16)
NF-kappa B signaling is required for the maintenance of normal B lymphocytes, whereas dysregulated NF-kappa B activation contributes to B cell lymphomas. The events that regulate NF-kappa B signaling in B lymphocytes are poorly defined. Here, we demonstrate that PKC-beta
Evolution of the eye transcriptome under constant darkness in Sinocyclocheilus cavefish.
Meng, F; Braasch, I; Phillips, JB; Lin, X; Titus, T; Zhang, C; Postlethwait, JH
Molecular Biology and Evolution null
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