05-983
Anti-PKC Antibody (α, β, γ), clone M110
clone M110, Upstate®, from mouse
别名:
PRKCA, PRKCB, PRKCG, protein kinase C, alpha, beta, gamma
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
M110, monoclonal
Application:
ELISA
immunocytochemistry
immunoprecipitation (IP)
western blot
immunocytochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
rat, human, mouse
Citations:
17
Technique(s):
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
产品名称
Anti-PKC Antibody (α, β, γ), clone M110, clone M110, Upstate®, from mouse
Quality Level
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
M110, monoclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PRKCA(5578)
Analysis Note
Routinely evaluated by immunoblot.
Application
Anti-PKC (α Antibody, β, γ), clone M110 detects level of PKC (α & has been published & validated for use in ELISA, IP, WB & IC.
Biochem/physiol Actions
Recognizes PKC α, β, γ. Does not cross react with other PKC isoforms.
General description
80-82 kDa
Immunogen
Recombinant protein of human PKCγ amino acids 499-697.
Physical form
100 μL of protein A purified mouse monoclonal IgG1 in 50% affinity storage buffer (PBS containing 1.0 mg/mL BSA and 0.05% sodium azide) and 50% glycerol.
Format: Purified
Preparation Note
Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Lilian Kisiswa et al.
Open biology, 7(1) (2017-01-20)
Tumour necrosis factor receptor 1 (TNFR1)-activated TNFα reverse signalling, in which membrane-integrated TNFα functions as a receptor for TNFR1, enhances axon growth from developing sympathetic neurons and plays a crucial role in establishing sympathetic innervation. Here, we have investigated the
Amos M Sakwe et al.
The Journal of biological chemistry, 280(6), 4436-4441 (2004-12-02)
This study examined the mechanism of Ca2+ entry and the role of protein kinase C (PKC) in Ca2+ signaling induced by activation of the calcium sensing receptor (CaR) in HEK293 cells stably expressing the CaR. We demonstrate that influx of
Michael Rosenberg et al.
Molecular biology of the cell, 17(3), 1364-1374 (2006-01-06)
Nonmuscle myosin II is an important component of the cytoskeleton, playing a major role in cell motility and chemotaxis. We have previously demonstrated that, on stimulation with epidermal growth factor (EGF), nonmuscle myosin heavy chain II-B (NMHC-IIB) undergoes a transient
Thomas T Su et al.
Nature immunology, 3(8), 780-786 (2002-07-16)
NF-kappa B signaling is required for the maintenance of normal B lymphocytes, whereas dysregulated NF-kappa B activation contributes to B cell lymphomas. The events that regulate NF-kappa B signaling in B lymphocytes are poorly defined. Here, we demonstrate that PKC-beta
Evolution of the eye transcriptome under constant darkness in Sinocyclocheilus cavefish.
Meng, F; Braasch, I; Phillips, JB; Lin, X; Titus, T; Zhang, C; Postlethwait, JH
Molecular Biology and Evolution null
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