The inner membrane of the nuclear envelope is connected to the nuclear lamina. The main components of the nuclear lamina are Type V intermediate filaments called lamins and lamin binding proteins including lamin B receptor, emerin, MANI, LAP1 (3 isoforms), and LAP2 (several isoforms). The lamins are divided into two groups: the constitutively expressed lamin B and the developmentaly regulated lamin A.
The best characterized isoforms of lamin binding protein LAP2 are LAP2α and LAP2β. LAP2β is a type II membrane protein in the inner nuclear membrane that binds lamin B and is important to cell viability and controlling nuclear lamina growth. LAP2α has been characterized as a nucleoplasmic protein that interacts with A-type lamins to control gene expression, transcription, and chromatin organization.

~55 kDa

KLH-conjugated synthetic linear peptide corresponding to LAP2.

Anti-LAP2 Antibody is an antibody against LAP2 for use in WB, IC & IF.

Immunofluorescence:
Representative lot data. This antibody was used to detect the nuclear lamina by immunofluorescence.

Immunocytochemistry:
Representative lot data.
A previous lot was used in confocal fluorescent analysis of A431, HeLa and NIH/3T3 cells using anti-LAP2 rabbit polyclonal antibody (Red).
Actin filaments have been labeled with AlexaFluor 488 -Phalloidin (Green). Nuclear is stained with DAPI (Blue). Positive nuclear staining.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cytoskeletal Signaling

Predicted to cross-react with canine (dog) and rat based on sequence homology.

This antibody recognizes LAP2.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, (pH 7.4), 150 mM NaCl, with 0.05% sodium azide.

Stable for 1 year at 2-8ºC from date of receipt.

Control
HeLa cell lysate

Evaluated by western blot on HeLa cell lysate.

Western Blot Analysis:
A 1:1000-1:3000 dilution of this antibody was used to detect LAP2 in HeLa cell lysate.

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