06-1106
Anti-Sonic Hedgehog Antibody
from rabbit, purified by affinity chromatography
别名:
sonic hedgehog, sonic hedgehog (Drosophila) homolog, sonic hedgehog homolog (Drosophila)
产品名称
Anti-Sonic Hedgehog Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SHH(6469)
Other Notes
Replaces: 04-971
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
General description
~ 45 kDa
Sonic Hedgehog Protein (SHH, VHH-1) belongs to hedgehog protein family which includes Indian Hh, and Desert Hh. Hh family is involved in the cell fate and patterning during embryonic development, homeostasis, and adult tissue renewal. Similar to other member in the family, SHH binds to the patched (PTC) cell surface receptor, releasing the signal transducer Smoothened (Smo) to transmit the Hh signal into the cell and activate transcription of the target gene. Precursor SHH is autocatlytically cleaved into two subunits, N-terminal and C-terminal products. Soluble N-terminal product is involved in the signaling activity, while C-product displays an autoproteolysis activity on the precursor and a cholesterol transferase activity on the N-terminal product. C-terminal product attaches a cholesterol moiety to the N-terminal product, preventing N-terminal product diffusion within the developing embryo. A defect in SHH has been linked in holoprosencephaly type 3 (HPE3), in which developing forebrain fails to separate into right and left hemispheres, and ocular coloboma.
Immunogen
Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of Sonic HedgeHog.
Analysis Note
Control
Human fetal skeletal muscle tissue lysate
Human fetal skeletal muscle tissue lysate
Evaluated by Western Blot in human fetal skeletal muscle tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate.
Application
Anti-Sonic Hedgehog Antibody detects level of Sonic Hedgehog & has been published & validated for use in WB, IH.
Research Category
Signaling
Signaling
Research Sub Category
Developmental Signaling
Developmental Signaling
Western Blot (SNAP ID) Analysis: 1 µg/mL from a previous lot detected Sonic Hedgehog on 10 µg of human fetal skeletal muscle tissue lysate.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Sonic Hedgehog in colorectal carcinoma tissue.
Immunohistochemistry Analysis: 1:500 dilution from a previous lot detected Sonic Hedgehog in colorectal carcinoma tissue.
Biochem/physiol Actions
This antibody recognizes the N-terminus of Sonic Hedgehog.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Gump, JM; Donson, AM; Birks, DK; Amani, VM; Rao, KK; Griesinger et al.
Acta Neuropathologica Communications null
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Genetics in medicine : official journal of the American College of Medical Genetics, 22(1), 189-198 (2019-08-10)
Preaxial polydactyly (PPD) is a common congenital hand malformation classified into four subtypes (PPD I-IV). Variants in the zone of polarizing activity regulatory sequence (ZRS) within intron 5 of the LMBR1 gene are linked to most PPD types. However, the
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Sonic hedgehog (SHH) is abundantly expressed and critical for morphogenesis in embryonic lungs; however, SHH expression drops to a much lower level in mice from E17.5 and in humans from the 21st gestational week. We find that SHH expression is
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