产品名称
Anti-JAK2 Antibody, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, human, rat
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... JAK2(3717)
mouse ... Jak2(16452)
rat ... Jak2(24514)
Analysis Note
Control
RAW264.7 cell lysate
RAW264.7 cell lysate
Evaluated by Western Blot in RAW264.7 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected JAK2 in 10 µg of RAW264.7 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected JAK2 in 10 µg of RAW264.7 cell lysate.
Application
Research Category
Inflammation & Immunology
Signaling
Inflammation & Immunology
Signaling
Research Sub Category
Immunological Signaling
Kinases & Phosphatases
Immunological Signaling
Kinases & Phosphatases
This Anti-JAK2 Antibody is validated for use in WB, IP for the detection of JAK2.
Western Blot Analysis: 1 µg/mL from a previous lot detected JAK2 in 10 µg of L6 cell lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JAK2 from RAW264.7 cell lysate.
Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JAK2 from RAW264.7 cell lysate.
Biochem/physiol Actions
This antibody recognizes the protein kinase 1 domain of JAK2.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The Janus family of tyrosine kinases (JAK) includes JAK1, JAK2, JAK3 and Tyk2. This family associates with a variety of cytokine receptors and is activated by ligand binding. Upon ligand binding to cytokine receptors, JAKs phosphorylate themselves and their associated receptors. This provides multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including STATs, Shc, insulin receptor substrates and FAK.
~130 kDa
Immunogen
Epitope: Protein kinase 1 domain
KLH-conjugated linear peptide corresponding to the protein kinase 1 domain of mouse JAK2.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein A purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Sandrine Medves et al.
Haematologica, 96(10), 1406-1414 (2011-06-21)
KANK1-PDGFRB is a fusion gene generated by the t(5;9) translocation between KANK1 and the platelet-derived growth factor receptor beta gene PDGFRB. This hybrid was identified in a myeloproliferative neoplasm featuring severe thrombocythemia, in the absence of the JAK2 V617F mutation.
Jiahai Shi et al.
Experimental hematology, 44(11), 1044-1058 (2016-10-27)
JAK2 V617F is a mutant-activated JAK2 kinase found in most polycythemia vera (PV) patients; it skews normal proliferation and differentiation of hematopoietic stem and progenitor cells and simulates aberrant expansion of erythroid progenitors. JAK2 V617F is known to activate some
Xiaomei Li et al.
Virology, 551, 75-83 (2020-08-25)
Porcine epidemic diarrhea (PED) virus (PEDV) is a coronavirus that primarily infects porcine intestinal epithelial cells and causes severe diarrhea and high fatality in piglets. A77 1726 is the active metabolite of leflunomide, a clinically approved anti-rheumatoid arthritis (RA) drug.
Alice H Grant et al.
International journal of molecular sciences, 24(7) (2023-04-14)
Overactive Janus kinases (JAKs) are known to drive leukemia, making them well-suited targets for treatment. We sought to identify new JAK-activating mutations and instead found a JAK1-inactivating pseudokinase mutation, V666G. In contrast to other pseudokinase mutations that canonically lead to
Ting Wang et al.
EMBO molecular medicine, 9(8), 1150-1164 (2017-06-03)
The endocrine system is crucial for maintaining whole-body homeostasis. Little is known regarding endocrine hormones secreted by the heart other than atrial/brain natriuretic peptides discovered over 30 years ago. Here, we identify growth differentiation factor 15 (GDF15) as a heart-derived hormone
相关内容
Pathways and Biomarkers of JAK/STAT Signaling
The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia.
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