06-1328
Anti-Themis Antibody
from rabbit, purified by affinity chromatography
别名:
thymocyte selection associated, thymocyte selection pathway associated, chromosome 6 open reading frame 190, chromosome 6 open reading frame 207, protein THEMIS, Thymocyte-expressed molecule involved in selection
产品名称
Anti-Themis Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse
species reactivity (predicted by homology)
rat (73% sequence homology)
technique(s)
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Themis(210757)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Analysis Note
Control
Mouse thymocyte tissue lysate
Mouse thymocyte tissue lysate
Evaluated by Western Blot in mouse thymocyte tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate.
Application
Detect Themis using this Anti-Themis Antibody validated for use in WB, FC.
Biochem/physiol Actions
This antibody recognizes Themis at the C-terminus.
General description
Thymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells.
~72 kDa observed.
UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa
UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa
Immunogen
KLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Mukul Prasad et al.
Cellular & molecular immunology, 18(9), 2249-2261 (2020-11-13)
Themis is a T cell lineage-specific molecule that is involved in TCR signal transduction. The effects of germline Themis deletion on peripheral CD4+ T cell function have not been described before. In this study, we found that Themis-deficient CD4+ T
Differential function of Themis CABIT domains during T cell development.
Okada, T; Nitta, T; Kaji, K; Takashima, A; Oda, H; Tamehiro, N; Goto, M; Okamura et al.
Testing null
Namrata Gautam et al.
Life science alliance, 6(12) (2023-09-23)
Themis is important in regulating positive selection of thymocytes during T cell development, but its role in peripheral T cells is less understood. Here, we investigated T cell activation and its sequelae using a tamoxifen-mediated, acute Themis deletion mouse model.
Themis sets the signal threshold for positive and negative selection in T-cell development.
Fu, G; Casas, J; Rigaud, S; Rybakin, V; Lambolez, F; Brzostek, J; Hoerter, JA; Paster et al.
Nature null
Joanna Brzostek et al.
Nature immunology, 21(2), 186-198 (2020-01-15)
T cell homeostasis and functional responsiveness require signals from self-peptide-major histocompatibility complex (self-pMHC) and cytokines, but the mechanisms controlling this signal integration are unknown. Using a conditional deletion of the T cell lineage-specific protein Themis, we show that Themis is
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