生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
serum
克隆
polyclonal
种属反应性
mouse, human
制造商/商品名称
Upstate®
技术
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... GNB2(2783)
一般描述
35/36kDa
免疫原
Internal sequence of G-protein β subunit (KTREGNVRVSREL) conjugated to octavalent polylysine base
应用
Detect Gβ using this Anti-Gβ Antibody validated for use in IP & WB.
生化/生理作用
Gβ subunits; recognizes both isoforms
外形
PBS, pH 7.2
分析说明
Immunoblot Analysis: A 1:500-1:1000 dilution of this antibody detected the Gβ subunits in 20 μg of a cell lysate prepared from rat brain microsomal preparation (Catalog # 12-144).
Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).
Immunoprecipitation/Immunoblot: 10 μl of this antibody immunoprecipitated the Gβ subunit from 500 μg-1mg of a lysate of rat brain microsomal preparation (Catalog # 12-144).
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 2
J Yamauchi et al.
The Journal of biological chemistry, 275(11), 7633-7640 (2000-03-14)
Heterotrimeric G proteins stimulate the activities of two stress-activated protein kinases, c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinase in mammalian cells. In this study, we examined whether alpha subunits of G(i) family activate JNK using transient expression system
M C Galas et al.
The Journal of biological chemistry, 272(5), 2788-2793 (1997-01-31)
The ADP-ribosylation factor (ARF) GTP-binding proteins are believed to function as regulators of vesicular budding and fusion along the secretory pathway. To investigate the role of ARF in regulated exocytosis, we have examined its intracellular distribution in cultured chromaffin cells
Importance of the G protein gamma subunit in activating G protein-coupled inward rectifier K(+) channels.
T Kawano, L Chen, S Y Watanabe, J Yamauchi, Y Kaziro, Y Nakajima, S Nakajima, H Itoh
Febs Letters null
H I Krieger-Brauer et al.
The Journal of biological chemistry, 272(15), 10135-10143 (1997-04-11)
Human fat cells possess a multireceptor-linked H2O2-generating system that is activated by insulin. Previous studies revealed that manganese was the sole cofactor required for a hormonal regulation of NADPH-dependent H2O2 generation in vitro. In this report it is shown that
T Yamaguchi et al.
The Journal of biological chemistry, 272(40), 25260-25266 (1997-10-06)
Nordihydroguaiaretic acid (NDGA) caused disassembly of the Golgi apparatus of NRK cells in a dose-, time-, and energy-dependent manner but not in a microtubule-dependent manner. In contrast to brefeldin A, NDGA did not cause release of beta-COP, a component of
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