biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rabbit, human, rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CDKN1B(1027)
相关类别
General description
27kda
p27（Kip1）是一种细胞周期蛋白依赖性激酶抑制剂，参与 G1 阻滞。p27（Kip1）结合并抑制 cyclinE-Cdk2 复合物、cyclinA-CDK2 和 cyclinD1-CDK4。p27（Kip1）受丝氨酸 10（S10）和苏氨酸 187（T187）磷酸化的调控。CDK2 磷酸化 T187 导致 p27（Kip1）的泛素化和降解。而 hKIS 磷酸化 S10 则导致出现从胞核向胞质转运的信号。
Immunogen
与 KLH 连接的 18 个氨基酸的合成肽（[C] GSVEQTPKKPGLRRRQT）已用于免疫兔
Application
抗 Kip1（p27）抗体是一种高质量的兔多克隆抗体，用于 Kip1（p27）的检测，&已在 IHC、IP & WB中得到验证。
Biochem/physiol Actions
识别 Kip1。
Physical form
形式：纯化的
Analysis Note
对照
阳性抗原对照：货号12-309，HeLa细胞核提取物。添加 等体积Laemmli样品还原性缓冲液至10 μL提取物并 煮沸5分钟以还原制剂。在小型凝胶每个泳道上样20 μg 经还原的提取物。
阳性抗原对照：货号12-309，HeLa细胞核提取物。添加 等体积Laemmli样品还原性缓冲液至10 μL提取物并 煮沸5分钟以还原制剂。在小型凝胶每个泳道上样20 μg 经还原的提取物。
通过免疫印迹对 HeLa 核提取物或兔脑核提取物进行了常规评估
Other Notes
替代：04-240
浓度：请参考批次特异性浓缩物的分析证书。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Protein phosphatase PP6 N terminal domain restricts G1 to S phase progression in human cancer cells.
Bjarki Stefansson et al.
Cell cycle (Georgetown, Tex.), 6(11), 1386-1392 (2007-06-15)
Yeast SIT4 is an essential gene encoding a protein Ser/Thr phosphatase conserved throughout eukaryotic evolution, known as PPV in Drosophila and PP6 in vertebrates. Sit4 promotes transcription of G1 cyclins and a sit4(ts) strain exhibits a G1 arrest at the
High-throughput screening reveals alsterpaullone, 2-cyanoethyl as a potent p27Kip1 transcriptional inhibitor.
Walters, BJ; Lin, W; Diao, S; Brimble, M; Iconaru, LI; Dearman, J; Goktug, A; Chen, T; Zuo, J
Testing null
Masahiro Morita et al.
Molecular and cellular biology, 27(13), 4980-4990 (2007-04-25)
The stability of mRNA influences the abundance of cellular transcripts and proteins. Deadenylases play critical roles in mRNA turnover and thus are important for the regulation of various biological events. Here, we report the identification and characterization of CCR4b/CNOT6L, which
Cell cycle. Stifled by inhibitions.
Peters, G
Nature, 371, 204-205 (1994)
Forkhead transcription factor FoxO1 transduces insulin-like growth factor's signal to p27Kip1 in primary skeletal muscle satellite cells.
Shuichi Machida, Espen E Spangenburg, Frank W Booth
Journal of Cellular Physiology null
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