生物来源
rabbit
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, Xenopus, rat, mouse
制造商/商品名称
Upstate®
技术
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
dry ice
靶向翻译后修饰
phosphorylation (pSer463/pSer465)
基因信息
human ... SMAD1(4086)
一般描述
65 kDa
Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), function downstream of TGF-β receptor Serine/Threonine kinases and undergo serine phosphorylation in response to receptor activation. Following BMP (bone morphogenic protein) or TGF-β binding to the targeted surface receptors, Smad1 (also designated Madr1 or JV4-1) becomes phosphorylated at Ser 463 and Ser 465. Activated Smad1 associates with Smad4 and translocates to the nucleus, where the Smad1/Smad4 complex recruits DNA-binding proteins to activate specific gene transcription. BMP binding induces phosphorylation of Smad1, which enhances the binding of Smad1 to CBP to stimulate Smad1-dependent transcription. In addition, ALK1 and ALK2 specifically phosphorylate Smad1 on residues located in the MH2 domain and induces Smad1-dependent pathways.
免疫原
peptide corresponding to (CGGSPHNPISpSVpSGG) corresponding to amino acids 455-465 of human Smad1 protein
应用
Detect phospho-Smad1 (Ser463/465) with Anti-phospho-Smad1 (Ser463/465) Antibody (Rabbit Polyclonal Antibody), that has been shown to work in WB.
生化/生理作用
Recognizes the dual serine phosphorylated Smad1 (Ser463/465).
外形
Format: Purified
分析说明
Control
BMP-4-treated HeLa or NIH/3T3 cells
BMP-4-treated HeLa or NIH/3T3 cells
routinely evaluated by immunoblot on RIPA lysates from P19 (mouse embryonal carcinoma) cells treated with BMP4
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
C Gruendler et al.
The Journal of biological chemistry, 276(49), 46533-46543 (2001-09-26)
The bone morphogenetic proteins (BMPs) regulate early embryogenesis and morphogenesis of multiple organs, such as bone, kidney, limbs, and muscle. Smad1 is one of the key signal transducers of BMPs and is responsible for transducing receptor activation signals from the
Tomoko Watanabe et al.
Development (Cambridge, England), 145(20) (2018-10-07)
The pre-placodal ectoderm (PPE) is a specialized ectodermal region which gives rise to the sensory organs and other systems. The PPE is induced from the neural plate border during neurulation, but the molecular mechanism of PPE formation is not fully
Bone morphogenetic protein signaling and growth suppression in colon cancer.
Beck, SE; Jung, BH; Fiorino, A; Gomez, J; Rosario, ED; Cabrera, BL; Huang, SC; Chow et al.
American Journal of Physiology: Gastrointestinal and Liver Physiology null
BMP and activin receptor expression in lens development.
Robbert U de Iongh, Yongjuan Chen, Maria I Kokkinos, John W McAvoy, Robbert U de Iongh et al.
Molecular Vision null
Xiaodan Yu et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 35(8), 1671-1682 (2016-10-22)
Increasing evidence supports the idea that bone morphogenetic proteins (BMPs) regulate cartilage maintenance in the adult skeleton. The aim of this study is to obtain insight into the regulation of BMP activities in the adult skeletal system. We analyzed expression
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