FAS-associated death domain protein (UniProt: Q13158; also known as FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD) is encoded by the FADD (also known as MORT1, GIG3) gene (Gene ID: 8772) in human. FADD is an apoptotic adaptor molecule that bridges death receptor signaling to the caspase cascade. It is expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. FADD contains a death domain at its C-terminus and a death effector domain (DED) at its N-terminal. The death domain is responsible for FADD binding to the activated trimerized Fas receptor. FADD recruits procaspase-8 through its N-terminal DED, which is then activated following the cleavage of pro domain. The death inducing signaling complex (DISC) formed by Fas receptor, FADD, and caspase-8 is essential in triggering apoptotic events in cells. Human FADD is phosphorylated mainly at Ser194. Defects in FADD gene have been linked to recurrent infections, hepatic dysfunction, and cardiovascular malformations. These subjects display high circulating levels of CD4(-) and CD8(-) cells and elevated levels of IL-10.

~26 kDa observed; 23.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

His-tagged full-length human recombinant FAS-associated death domain protein.

Detect FAS-associated death domain protein using this rabbit polyclonal Anti-FADD, Cat. No. 06-711. It has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected FADD in human pancreas, human kidney, and human prostate tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This polyclonal antibody detects FADD protein in human cells.

ImmunoAffinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected FADD in 10 µg of Jurkat cell lysate.

Concentration: Please refer to lot specific datasheet.

Replaces: 04-1113

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