生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
E. coli
制造商/商品名称
Upstate®
技术
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
Escherichia coli K12 ... Lexa(948544)
一般描述
24 kDa
抗LexA抗体可检测LexA蛋白和与LexA蛋白融合的重组蛋白。该抗体可用于检测构建用于酵母双杂交系统或其他相互作用捕获系统的潜在诱饵蛋白的表达。LexA会增加约22至25 kDa的蛋白质，具体取决于额外氨基酸的数量。
免疫原
对应于氨基酸残基1-202的LexA蛋白的带有His标签的DNA结合域
应用
用抗LexA抗体，DNA结合区（兔多克隆抗体）检测LexA，该抗体已显示可在IP & WB中起作用。
生化/生理作用
识别酵母双杂交系统中使用的诱饵融合蛋白的LexA部分。
外形
形式：纯化
分析说明
对照
LexA-pRB转染酵母细胞提取物
LexA-pRB转染酵母细胞提取物
通过免疫印迹对LexA-pRB转染酵母裂解液进行了常规评估
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Vanessa C Fernandes et al.
The Journal of biological chemistry, 294(15), 5980-5992 (2019-02-16)
Genetic testing for BRCA1, a DNA repair protein, can identify carriers of pathogenic variants associated with a substantially increased risk for breast and ovarian cancers. However, an association with increased risk is unclear for a large fraction of BRCA1 variants
Getting more from the two-hybrid system: N-terminal fusions to LexA are efficient and sensitive baits for two-hybrid studies.
Beranger, F, et al.
Nucleic Acids Research, 25, 2035-2036 (1997)
Direct identification of insulator components by insertional chromatin immunoprecipitation.
Fujita, T; Fujii, H
Testing null
Yu-Fan Chen et al.
Molecular and cellular biology, 36(15), 2039-2050 (2016-05-18)
Cohesin associates with distinct sites on chromosomes to mediate sister chromatid cohesion. Single cohesin complexes are thought to bind by encircling both sister chromatids in a topological embrace. Transcriptionally repressed chromosomal domains in the yeast Saccharomyces cerevisiae represent specialized sites
Notch signaling is antagonized by SAO-1, a novel GYF-domain protein that interacts with the E3 ubiquitin ligase SEL-10 in Caenorhabditis elegans.
Hale, VA; Guiney, EL; Goldberg, LY; Haduong, JH; Kwartler, CS; Scangos, KW; Goutte, C
Genetics null
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