产品名称
抗-FcεRI抗体,γ 亚基, Upstate®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FCER1A(2205)
Application
研究类别
炎症 & 免疫学
炎症 & 免疫学
抗FceRI,γ亚基(目录号06-727)是一种兔多克隆抗体,可检测FceRI亚基,并已测试可用于流式细胞术和蛋白质印迹。
流式细胞分析:从代表性批次中取0.1 µg在大鼠嗜碱性白血病细胞中检测到FceRI,γ亚基。
研究子类别
免疫球蛋白 & 免疫学
免疫球蛋白 & 免疫学
Biochem/physiol Actions
与其他物种的交叉反应性还未经测试。
可识别并特异于FcεRI,γ-亚基,MW 10kDa。
Analysis Note
对照
RBL细胞裂解液
RBL细胞裂解液
已通过蛋白质印迹对RBL细胞的RIPA裂解液进行了评估。
蛋白质印迹分析:0.5-2 μg/mL该抗体在RBL细胞的RIPA裂解液中检测到FcεRI, γ-亚基。
蛋白质印迹分析:0.5-2 μg/mL该抗体在RBL细胞的RIPA裂解液中检测到FcεRI, γ-亚基。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
30 kDa
高亲和力免疫球蛋白ε受体亚基γ(UniProt P20411;也称为Fc-εRI-γ,Fc受体γ-链,FceRIγ,FcRγ,IgE Fc受体亚基γ)由大鼠物种中Fcer1g(也称为Fce1g)基因(基因ID:25441)编码。FceR1γ是一种单程I型膜蛋白,可与多种FcRα链结合形成功能性信号复合物,可调节免疫反应的多个方面。γ亚基在使IgE Fc受体到达细胞表面方面具有关键作用。FceRIγ仅在肥大细胞和嗜碱性粒细胞上发现。在啮齿动物中,FcεRI是非共价连接的亚基的四聚体复合物:一个IgE结合α亚基,一个β亚基和二硫键连接的γ亚基的二聚体。当多价过敏原与受体结合的IgE结合时,随之而来的受体聚集会导致引起过敏症状的介质释放。(参考文献:Blank, U., et al. (1989).Nature 337; 6203:187-189.
Immunogen
KLH缀合的线性肽,对应大鼠FceRI γ亚基的C端区域的7个氨基酸。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Physical form
形式:纯化
纯化的兔多克隆IgG,溶于含有70%储存缓冲液(0.1 M Tris-甘氨酸(pH 7.4)、0.15 M NaCl、0.05%叠氮化钠)和30%甘油的缓冲液中。
蛋白A纯化
Preparation Note
自收到之日起可稳定保存1年。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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