产品名称
Anti-IRAK Antibody, Upstate®, from rabbit
isotype
IgG
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
mouse ... Clec1B(56760)
Analysis Note
Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
routinely evaluated by immunoblot on HeLa nuclear extract
Application
Detect IRAK using this Anti-IRAK Antibody validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
Immunological Signaling
Immunological Signaling
Biochem/physiol Actions
Recognizes IRAK.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
80 kDa
Immunogen
Peptide (DRQGPEESDEFQS) corresponding to amino acids 700-712 of human IRAK
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Ammonium sulfate precipitation and DEAE-cellulose chromatography
Format: Purified
Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
Characterization of interleukin-1 receptor-associated kinase in normal and endotoxin-tolerant cells.
L Li et al.
The Journal of biological chemistry, 275(30), 23340-23345 (2000-05-16)
Interleukin-1 receptor-associated kinase (IRAK), a signal transducer for interleukin-1, has also been suggested to participate in the Toll-like receptor-mediated innate immune response to bacterial endotoxin lipopolysaccharide (LPS). Using the human promonocytic THP-1 cell line, we demonstrated that the endogenous IRAK
Cigarette smoke induces CXCL8 production by human neutrophils via activation of TLR9 receptor.
Mortaz, E; Adcock, IM; Ito, K; Kraneveld, AD; Nijkamp, FP; Folkerts, G
The European Respiratory Journal null
Jean Hu et al.
Journal of immunology (Baltimore, Md. : 1950), 168(8), 3910-3914 (2002-04-09)
IL-1R-associated kinase (IRAK) plays a pivotal role in IL-1R/Toll-like receptor (TLR)-mediated signaling and NF-kappaB activation. IRAK from leukocytes undergoes rapid activation and inactivation/degradation following IL-1 or LPS stimulation. The rapid degradation of IRAK may serve as a negative feedback mechanism
Candida albicans triggers activation of distinct signaling pathways to establish a proinflammatory gene expression program in primary human endothelial cells.
Verena Muller, Dorothee Viemann, Marc Schmidt, Nicole Endres, Stephan Ludwig et al.
Journal of immunology (Baltimore, Md. : 1950) (1950)
M Trofimova et al.
The Journal of biological chemistry, 271(30), 17609-17612 (1996-07-26)
The NF-kappaB/c-Rel proteins are a family of evolutionarily conserved transcription factors activated during development that in the adult, mediate many processes including the immune response. A high degree of sequence similarity is shared between the NF-kappaB/c-Rel family of transcription factors
相关内容
"Toll-like Receptors (TLRs) are transmembrane proteins that are expressed on various immune cells. The extracellular N-terminal region of TLRs recognizes specific pathogen components. At least 13 different members of TLR family have been identified that detect different pathogen associated molecular patterns (PAMPs), including lipopolysaccharides, flagellin, bacterial CpG DNA, and viral RNA and DNA. Recognition of PAMPs by TLRs is considered as a key process for the induction of an inflammatory response."
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