Silent synapses, or excitatory synapses that lack functional -amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs), are thought to be critical for regulation of neuronal circuits and synaptic plasticity. SynGAP, an excitatory synapse-specific RasGAP, regulates AMPAR trafficking, silent synapse number, and excitatory synaptic transmission in hippocampal and cortical cultured neurons. Over expression of SynGAP in neurons results in a remarkable depression of AMPAR-mediated miniature excitatory postsynaptic currents, a significant reduction in synaptic AMPAR surface expression, and a decrease in the insertion of AMPARs into the plasma membrane. Synaptic transmission is increased in neurons from SynGAP knockout mice as well as in neuronal cultures treated with SynGAP small interfering RNA.

SynGAP

peptide (KRLLDAQRGSFPPWVQQTRV) corresponding to amino acids 1289-1308 of rat SynGAP-a

Anti-SynGAP Antibody detects level of SynGAP & has been published & validated for use in IC & WB.

Immunocytochemistry: 0.1 µg/mL has been reported to immunostain SynGAP in neuron cultures fixed with 4% paraformaldehyde, 4% sucrose and perme-abilized with 0.1% Triton X-100 (2).

Research Category
Neuroscience

Research Sub Category
Synapse & Synaptic Biology

Replaces: 04-1071

Protein A purified

Format: Purified

Purified in PBS with 0.05% NaN3 and 30% Glycerol

Maintain at -20°C in undiluted aliquots for up to 1 year after date of receipt.

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