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Merck
CN

06-991

Anti-PKCε Antibody

Upstate®, from rabbit

别名:

protein kinase C, epsilon

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
rat, human, mouse
Citations:
25
Technique(s):
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
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产品名称

Anti-PKCε Antibody, Upstate®, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... PRKCE(5581)

Biochem/physiol Actions

Recognizes PKCε, Mr 95 kDa.
Predicted to cross-react with rabbit.

Immunogen

KLH-conjugated, synthetic peptide corresponding to the C-terminal variable (V5) region, amino acids 726-737 (KGFSYFGEDLMP), of mouse Protein Kinase C Epsilon (PKCε). The immunizing sequence is identical in human, mouse, rat and rabbit.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Approx. 95
Members of the protein kinase C family fall into three categories: conventional (PKC alpha, beta I, beta II, gamma), novel (delta, epsilon, eta, mu, theta), and atypical (lambda, zeta). Conventional PKC isoforms are dependent on calcium and diacylglycerol (DAG) for their activity, whereas novel PKC isoforms are calcium dependent, and atypical PKC isoforms are calcium and DAG independent.

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.
Routinely evaluated by Western Blot on Jurkat cell lysate.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected PKCε in Jurkat cell lysate. 0.5-2 μg/mL of a previous lot detected PKCε in a RIPA lysate from Jurkat cells, mouse brain extract and rat brain extract.

Application

Detect PKCε using this Anti-PKCε Antibody validated for use in IH, IP & WB.
Immunoprecipitation:
An independent lab has reported this antibody immunoprecipitates PKCε.

Immunohistochemistry:
An independent lab has reported this antibody detects PKCε.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol.
Store at -20°C.

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1


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Andressa G Amaral et al.
Pancreas, 39(6), 847-855 (2010-08-11)
We investigated the influence of leucine supplementation on insulin secretion and on some proteins related to insulin secretion in malnourished mice. Swiss mice (aged 21 days) received isocaloric normo-17% (NP) or 6% low-protein (LP) diet for 120 days. Half of
Two conventional protein kinase C isoforms, alpha and beta I, are involved in the ATP-induced activation of volume-regulated anion channel and glutamate release in cultured astrocytes.
Alena Rudkouskaya,Artur Chernoguz,Renee E Haskew-Layton,Alexander A Mongin
Journal of Neurochemistry null
Protein kinase C epsilon activates lens mitochondrial cytochrome c oxidase subunit IV during hypoxia.
Michael Barnett, Dingbo Lin, Vladimir Akoyev, Lloyd Willard, Dolores Takemoto et al.
Experimental Eye Research null
Platelet-activating factor induces cardioprotection in isolated rat heart akin to ischemic preconditioning: role of phosphoinositide 3-kinase and protein kinase C activation.
Penna, C; Alloatti, G; Cappello, S; Gattullo, D; Berta, G; Mognetti, B; Losano, G; Pagliaro, P
American Journal of Physiology. Heart and Circulatory Physiology null
Post-ischaemic activation of kinases in the pre-conditioning-like cardioprotective effect of the platelet-activating factor.
Penna C, Mognetti B, Tullio F, Gattullo D, Mancardi D, Moro F, Pagliaro P, Alloatti G
Acta Physiologica (Oxford, England) null

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