Anti-PP2A Antibody, Catalytic subunit

60 kDa

PP2A is a type 2 protein Ser/Thr phosphatase characterized by high activity against the α subunit of Phosphorylase Kinase and low sensitivity to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. PP2A is a heterotrimer containing a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B′/PR61, and B′′/PR72 . PTPA is a member of the B′ family that is required for activation of PP2A by an undefined mechanism.

Epitope: C-terminus

Synthetic peptide corresponding to a.a. 298-309 of the human sequence.

Immunohistochemisty (paraffin): Antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as staining in normal human testis.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

This Anti-PP2A Antibody, Catalytic subunit is validated for use in WB, IH(P) for the detection of PP2A.

Peptide is highly conserved in human, rat, bovine, mouse and chicken.

Recognizes protein phosphatase 2A, catalytic subunit.

Affinity purified

Purified rabbit polyclonal IgG in buffer containing PBS with no preservatives.

Stable for 1 year at -80°C from date of receipt. Avoid repeated freeze/thaw.

Control
HEK293 cell lysate

Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500-1:1,000 dilution is traditonally used to detected PP2A, Catalytic subunit in 10 μg of HEK293 lysates.

Replaces: AB1621

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