Recognizes the catalytic subunit of Protein Phosphatase 6 (PP6), MW ~33kDa

This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression.

The immunogen used to the purified peptide conjugated to KLH corresponding to the sequence NH2-Cys-Ala-Val-Pro-Asp-Ser-Glu-Arg-Val-Ile- Pro-Pro-Arg-Thr-Thr-Thr-Pro-Tyr-COOH.

Immunoprecipitation: A previous lot of this antibody was used in immunoprecipitation at a dilution of 10-20 µg/mL.
Immunohistochemistry(paraffin):
Optimal Staining of PP6 Polyclonal Antibody:
Human Testis
PP6 staining of round spermatids in normal human
testis, tissue pretreated with citrate buffer, pH 6.0.
Antibody was diluted 1:100, using IHC-Select
detection with HRP-DAB.

Detect PP6C using this Anti-PP6C Antibody validated for use in WB, IP, IH(P).

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Recognizes the catalytic subunit of Protein Phosphatase 6, MW ~33kDa.

Format: Purified

Purified rabbit polyclonal IgG in buffer containing PBS with 0.08% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Control
Jurkat Cell Lysate

Routinely evaluated by Western Blot on Jurkat lysates.

Western Blot Analysis: 1:500 dilution of this lot detected PP6C on 10 μg of Jurkat lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB4091

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.