Death Associated Protein Kinase (DAPK) is a Ca2+/Calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNFα and Interferon-γ. Deletion of the Calmodulin-binding domain results in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerts a dominant negative effect, reducing cytotoxicity and protecting cells from Interferon-γ induced cell death. DAPK expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlate with high rates of metastasis.

Detects a 42 kDa band by western blot.

Detects a 42 kDa band by western blot, corresponding to DAPK2. Does not cross-react with DAPK1.

Peptide corresponding to aa 356-370 (ARRKALHPRRRSSTS) of human DAPK2, which is identical to the sequence of mouse origin.

Detect DAPK2 using this Anti-DAPK2 Antibody validated for use in WB, IH(P).

Immunohistochemisty (paraffin): DAPK2 representative staining pattern/morphology in apoptotic bodies in colon cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.

Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.

Research Category
Signaling

Research Sub Category
GPCR, cAMP/cGMP & Calcium Signaling

Replaces: AB3606

Affinity purified

Purified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide.

Stable for 6 months at 2-8° C from date of receiept.

Control
C2C12 cell lysate

Routinely evaluated by Western Blot on C2C12 lysates.

Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.