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Merck
CN

07-1466

Cdc42抗体

from rabbit

别名:

G25K, GTP-binding Protein

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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产品名称

Cdc42抗体, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human, rat

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... CDC42(998)

Analysis Note

对照
大鼠脑裂解液
通过蛋白质印迹对大鼠脑裂解物进行常规评估。

蛋白质印迹分析:大鼠脑裂解液,使用先前批次兔抗Cdc42的1:500-1:2000(0.5-2μg/mL)稀释液。

Application

研究子类别
MAP激酶
研究类别
信号传导
蛋白质印迹分析:大鼠脑,使用之前批次的兔抗Cdc42(货号07-1466)和兔抗Rac1(货号07-14664)。
Rac1和Cdc42的分子量为~21 kDa。

最佳工作稀释度必须由最终用户确定。
这种Cdc42抗体经验证可用于WB法检测Cdc42。

Biochem/physiol Actions

可识别Cdc42,不与Rac1交叉反应。Cdc42是小GTP酶Rho家族的成员。 小GTP酶通过在GDP结合(非活化)态和GTP结合(活化)态之间循环,在调节各种生物反应途径中充当分子开关。 Cdc42诱导形成丝状伪足。 Cdc42可以激活Rac(因此,丝状伪足与板状伪足密切相关)。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

21 kDa
cdc42是小GTP结合蛋白Rho家族的成员,在细胞骨架肌动蛋白组织和转化中发挥作用。Rac在cdc42下游激活,或被Ras信号独立激活,Rac反过来刺激Rho。cdc42的激活刺激丝状伪足的形成,而Rac导致板状伪足的形成,Rho刺激肌动蛋白应力纤维的形成。cdc42/Rac通路的效应子包括NADPH氧化酶(Phox)和SAPK1/JNK激酶家族。后一条通路由PAK激酶激活,PAK激酶是cdc42的直接靶标。

Immunogen

对应于人Cdc42氨基酸130-141的合成肽
(AKNKQKPITPET-C)。

Other Notes

替代:AB4201
浓度:请参考批次特异性浓缩物的检验报告。

Physical form

亲和纯化
形式:纯化
纯化的兔IgG多克隆抗体,溶于含0.15 M NaCl、pH 7.6的0.02 M PBS(含0.1%叠氮化钠,作为防腐剂)中。

Preparation Note

收到货后,在2-8°C,以未稀释等分形式可最多稳定保存6个月

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Amanda Amoah et al.
Haematologica (2021-01-15)
In this study, we characterize age-related phenotypes of human hematopoietic stem cells (HSCs). We report increased frequencies of HSC, HPC and lineage negative cells in the elderly but a decreased frequency of multi-lymphoid progenitors. Aged human HSCs further exhibited a
Shiri Gur-Cohen et al.
Nature medicine, 21(11), 1307-1317 (2015-10-13)
Retention of long-term repopulating hematopoietic stem cells (LT-HSCs) in the bone marrow is essential for hematopoiesis and for protection from myelotoxic injury. We report that signaling cascades that are traditionally viewed as coagulation related also control retention of endothelial protein
Edward O Kwarteng et al.
Journal of leukocyte biology, 103(3), 381-393 (2018-01-19)
The switch from fetal to adult hematopoietic stem/progenitor cells (HSPCs) is associated with profound changes in several genetic programs. Although HSPC ageing corresponds to alterations in Wnt signaling, relatively little is known about the relative roles of different Wnt signaling
Tione Buranda et al.
Analytical biochemistry, 442(2), 149-157 (2013-08-10)
We describe a rapid assay for measuring the cellular activity of small guanine triphosphatases (GTPases) in response to a specific stimulus. Effector-functionalized beads are used to quantify in parallel multiple GTP-bound GTPases in the same cell lysate by flow cytometry.
Yang Liu et al.
Developmental biology, 383(2), 239-252 (2013-09-24)
Neural crest cells (NCCs) are physically responsible for craniofacial skeleton formation, pharyngeal arch artery remodeling and cardiac outflow tract septation during vertebrate development. Cdc42 (cell division cycle 42) is a Rho family small GTP-binding protein that works as a molecular

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