07-2113
Anti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244)
from rabbit, purified by affinity chromatography
别名:
40S ribosomal protein S6, Phosphoprotein NP33
产品名称
Anti-phospho-Ribosomal Protein S6 Antibody (Ser240/Ser244), from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer240/pSer244)
Quality Level
Gene Information
human ... RPS6(6194)
Analysis Note
Control
PMA treated and untreated A431 cell lysates
PMA treated and untreated A431 cell lysates
Evaluated by Western Blot in PMA treated and untreated A431 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected Ribosomal Protein S6 on 10 µg of PMA treated and untreated A431 cell lysates.
Western Blot Analysis: 2 µg/mL of this antibody detected Ribosomal Protein S6 on 10 µg of PMA treated and untreated A431 cell lysates.
Application
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Ribosomal Protein S6 in A431 cells and PMA treated A431 cells.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
This phospho-Ribosomal Protein S6 antibody is validated for use in WB & ICC for the detection of the phospho-Ribosomal Protein S6 protein.
Biochem/physiol Actions
This antibody recognizes Ribosomal Protein S6 phosphorylated at Ser240/Ser244.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
S6 (Ribosomal protein S6, RpS6, 40S ribosomal protein S6) is known to play a role in cell growth and proliferation. It lays downstream of both the PI3K/Akt/mTOR pathway as a substrate of p70 S6 kinase and the Ras/Erk pathway as a substrate of RSK. S6 is phosphorylated in response to stimulation by growth factors, tumor promoting agents, and mitogens. It is dephosphorylated at growth arrest. There are two major areas of S6 phosphorylation; Ser235/Ser236 that are known to be phosphorylated by both p70S6 Kinase as well as RSK and Ser240/244 which is thought to only be phosphorylated by p70S6K.
~32 kDa observed
Immunogen
Epitope: Phosphorylated Ser240/Ser244
KLH-conjugated linear peptide corresponding to human Ribosomal Protein S6 phosphorylated at Ser240/Ser244.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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