This protein is a member of the family of STAT proteins (Signal Transduction and Activators of Transcription). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. The Stat3 protein is expressed in most cell types and is activated by multiple cytokines and growth factors including: IFN-a, IL-10, IL-6, IL-11, onM, LIF, CNTF, G-CSF, IL-12, IL-2, GH, EGF, PDGF, and CSF-1. Stat3 is not activated by IFN beta. One active Stat3 translocates to the nucleus and binds to DNA as either a homodimer or as a heterodimer with Stat1. Transcription factors associated with Stat3 include cJun and cyclic AMP responsive enhancer binding protein (CREB). Stat3 interacts with the APRE (acute phase response element) and the SIE (c-sis inducible element). These proteins are latent cytoplasmic transcription factors with the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue.

~ 88 kDa observed.

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human STAT3 near the C-terminus.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Use Anti-Stat3 Antibody (rabbit polyclonal antibody) validated in WB, IP to detect Stat3 also known as Acute-phase response factor, DNA-binding protein APRF, signal transducer & activator of transcription 3.

Western Blot (SNAP ID) Analysis: 5 µg/mL from a representative lot detected STAT3 on 10 µg of HeLa cell lysate.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated STAT3 from 500 µg of HeLa RIPA lysate.

This antibody recognizes STAT3 near the C-terminus.

Protein A

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected STAT3 on 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB3162

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