Anti-CAPZIP Antibody

CAPZIP (CapZ-interacting protein) is highly expressed in immune cells and muscle and uniquely interacts with CapZ, an F-actin capping protein, in splenocytes. Phosphorylation of CAPZIP is thought to regulate CapZ ability to remodel actin filament assembly. CAPZIP may also contain a CPI (capping protein interaction) motif that seems to restrict capping protein to a low-affinity, filament-binding conformation.

~70 kDa observed. The native form of CAPZIP runs anomalously, with a molecular mass of 70 kDa (much slower than its real molecular mass). The 47 kDa band may be non-specific, a splice variant or a degradation product. (Eyers, C.E., et al. (2005). Biochem J. 389(1):127-135.). An uncharacterized band appears at ~18 kDa

GST-tagged recombinant protein corresponding to human CAPZIP.

Anti-CAPZIP detects levels of CAPZIP proteins & has been published & validated for use in WB & IP.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated CAPZIP from Jurkat cell lysate.

Other homologies: Mouse (64% sequence homology). Rat (58% sequence homology).

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Jurkat cell lysate

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 1:250 dilution of this antibody detected CAPZIP on 10 µg of Jurkat cell lysate.

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