Anti-WNK3 Antibody

WNK3 is a serine-threonine protein kinase and expressed throughout the nephron, but most commonly at intercellular junctions. In its active state, it is a promoter of NKCC2 and NCC activity. The opposite is true of WNK3 when in an inactive state. WNK3 changes the expression of these transporters in the membrane and is thought to be a regulator of blood pressure along with other members of the WNK family of protein kinases. In recent research, WNK3 has also been linked to regulating signaling cascades involved in tumorigenesis.

~220 kDa observed. Uncharacterized bands appear at ~52 and 60 kDa

GST-tagged recombinant protein corresponding to human WNK3.

Research Category
Signaling

Research Sub Category
Developmental Signaling

This WNK3 antibody is validated for use in WB for the detection of the WNK3 protein.

Other homologies: Rat (74% sequence homology).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HEK293 cell lysate

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected WNK3 on 10 µg of HEK293 cell lysate.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.