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Merck
CN

07-2276-I

Anti-mSin1 Antibody

from rabbit, purified by affinity chromatography

别名:

Target of rapamycin complex 2 subunit MAPKAP1, TORC2 subunit MAPKAP1, Mitogen-activated protein kinase 2-associated protein 1, Stress-activated map kinase-interacting protein 1, SAPK-interacting protein 1, mSIN1, mSin1

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunoprecipitation (IP)
western blot
Species reactivity:
rat, human, mouse
Citations:
1
Technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
Q9BPZ7

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产品名称

Anti-mSin1 Antibody, from rabbit, purified by affinity chromatography

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

rat, human, mouse

species reactivity (predicted by homology)

porcine (based on 100% sequence homology), feline (based on 100% sequence homology), horse (based on 100% sequence homology), hamster (based on 100% sequence homology), bovine (based on 100% sequence homology)

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NP_001006618

UniProt accession no.

Q9BPZ7

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... MAPKAP1(79109)

Analysis Note

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected mSin1 in 10 µg of A431 cell lysate.

Application

Detect MAPKAP1/Sin1 using this rabbit polyclonal Anti-mSin1 Antibody, Cat. No. 07-2276-I, validated for use in Western Blotting and Immunoprecipitation.
Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated mSin1 in 0.5 mg of A431 cell lysate.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

Biochem/physiol Actions

Expected to react with spliced isoforms 1, 2, 3, 5, 6, but not 4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Target of rapamycin complex 2 subunit MAPKAP1 (UniProt Q9BPZ7; also known as MEKK2-interacting protein 1, Mitogen-activated protein kinase 2-associated protein 1, mSIN1, Ras inhibitor MGC2745, SAPK-interacting protein 1, Stress-activated protein kinase-interacting 1, Stress-activated map kinase-interacting protein 1,, TORC2 subunit MAPKAP1) is encoded by the MAPKAP1 (also known as JC310, MIP1 SIN1, SIN1b, SIN1g) gene (Gene ID 79109) in human. mSIN1 is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase, along with mSIN1, GbL (mLST8), Protor1, Protor2, and Rictor. The mTORC2 complex is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is known to affect cytoskeletal organization and cell migration by exerting its effects through Rac, Rho, and PKC. mSIN1 is also known to directly interact with Ras, SPAKs, and JNK.
~59 kDa observed

Immunogen

Epitope: MAP3K2/NBN interaction domain
KLH-conjugated linear peptide corresponding to the MAP3K2/NBN interaction domain of human mSin1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Yasir H Ibrahim et al.
The Journal of biological chemistry, 299(5), 104644-104644 (2023-03-26)
The mechanistic target of rapamycin complex 1 (mTORC1) is a central regulator of mammalian cell growth that is dysregulated in a number of human diseases, including metabolic syndromes, aging, and cancer. Structural, biochemical, and pharmacological studies that have increased our

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White Paper: Further considerations of antibody validation and usage.

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