Anti-AUF1 Antibody

37, 40, 42, 45 kDa

A+U Rich RNA Binding Factor (AUF1) is comprised of four isoforms of 37, 40, 42, and 45 kDa. Although the role of each isoform has yet to be fully characterized, a direct correlation has been observed between each AUF1 isoform’s binding affinity and its RNA destabilizing activity toward different AREs (A + U rich element in the 3’ untranslated region), with the isoforms p37 and p42 being the most effective.

AUF1 is a bcl2 mRNA binding protein and potentially all its isoforms are able to form complexes with the bcl2 ARE. ARE mediated bcl2 mRNA downregulation during apoptosis involves AUF1 and suggest different roles for its four isoforms.

Purified AUF1

Anti-AUF1 Antibody is a high quality Rabbit Polyclonal Antibody for the detection of AUF1 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

RNA Binding Protein (RBP)

Recognizes AUF1 isoforms.

Protein A purified

Format: Purified

Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

routinely evaluated by immunoblot on RIPA lysates from HeLa nuclear extract or A431 cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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