CN
生物来源
rabbit
质量水平
抗体形式
purified immunoglobulin
antibody product type
primary antibodies
克隆
polyclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
target post-translational modification
unmodified
Gene Information
human ... RAF1(5894)
特异性
Raf-1
免疫原
peptide corresponding to amino acids 637-648 of human Raf-1.
应用
Anti-Raf-1 Antibody detects level of Raf-1 & has been published & validated for use in IP & WB.
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
质量
routinely evaluated by immunoblot in RIPA lysates from 3T3/A31 cell lysates
目标描述
70kDa
联系
Replaces: 04-412
外形
Protein A purified
0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
储存及稳定性
2 years at -20degC
分析说明
Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存分类代码
10 - Combustible liquids
WGK
WGK 2
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货号
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Dectin-1 directs T helper cell differentiation by controlling noncanonical NF-kappaB activation through Raf-1 and Syk.
Sonja I Gringhuis,Jeroen den Dunnen,Manja Litjens,Michiel van der Vlist,Brigitte Wevers et al.
Nature Immunology null
L Lin et al.
Cell death and differentiation, 13(11), 1982-1993 (2006-04-01)
Melanoma differentiation-associated gene-5 (mda-5) was the first molecule identified in nature whose encoded protein embodied the unique structural combination of an N-terminal caspase recruitment domain and a C-terminal DExD/H RNA helicase domain. As suggested by its structure, cumulative evidences documented
Kiril Bidzhekov et al.
Journal of cellular and molecular medicine, 11(6), 1395-1407 (2008-01-22)
Mouse embryonic endothelial progenitor cells (eEPCs) acquire a mature phenotype after treatment with cyclic adenosine monophosphate (cAMP), suggesting an involvement of Raf serine/threonine kinases in the differentiation process. To test this idea, we investigated the role of B-Raf and C-Raf
Design and discovery of small molecules targeting raf-1 kinase.
Lowinger, Timothy B, et al.
Current Pharmaceutical Design, 8, 2269-2278 (2002)
Jennifer A Clark et al.
The Journal of biological chemistry, 279(10), 9233-9247 (2003-12-13)
We have reported previously that protein kinase C (PKC) signaling can mediate a program of cell cycle withdrawal in IEC-18 nontransformed intestinal crypt cells, involving rapid disappearance of cyclin D1, increased expression of Cip/Kip cyclin-dependent kinase inhibitors, and activation of
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