Anti-monomethyl Histone H3 (Lys4) Antibody, Alexa Fluor^™ 488 Conjugate

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 07-436.

~17 kDa observed (Refer to Cat. No. 07-436)

Linear peptide corresponding to Histone H3 monomethylated at Lys4.

This Anti-monomethyl Histone H3 (Lys4) Antibody, Alexa Fluor^™ 488 Conjugate is validated for use in ICC for the detection of monomethyl Histone H3 (Lys4).

Purified rabbit Alexa Fluor^™ 488 conjugated antibody in buffer containing PBS with 15 mg/ml BSA and 0.1% Sodium Azide.

Evaluated by Immunocytochemisty Analysis in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected monomethyl Histone H3 (Lys4) in HeLa cells.

Alexa Fluor^™ is a registered trademark of Life Technologies.

ALEXA FLUOR is a trademark of Life Technologies