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Merck
CN

07-441-AF488

抗二甲基组蛋白H3(Lys9)抗体,Alexa Fluor 488 偶联物

from rabbit, ALEXA FLUOR 488

别名:

Histone H3.1t, H3/t, H3t, H3/g, dimethyl-Histone H3 (Lys9), AlexaFluor 488 Conjugate

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR 488
Clone:
polyclonal
Application:
ICC
Citations:
-
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biological source

rabbit

conjugate

ALEXA FLUOR 488

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), yeast (based on 100% sequence homology), chicken (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

dimethylation (Lys9)

Quality Level

Gene Information

human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)

General description

可观察到约17 kDa的条带
组蛋白H3是参与真核细胞染色质结构的五种主要组蛋白之一。H3具有一个主要的球状结构域和一个长N末端的尾巴,其与核糖体核小体′串珠′结构体的结构有关。组蛋白H3的N末端尾部从球状核小体核心突起,其可能发生几种不同类型的表观遗传修饰,从而影响细胞过程。这些修饰包括甲基或乙酰基与赖氨酸和精氨酸的共价连接,以及丝氨酸或苏氨酸的磷酸化。组蛋白H3变体(H3.1,H3.2和H3.3)与细胞状态的表观遗传记忆有关。H3的全基因组模式取决于氨基酸序列,并随着发育调控基因座的细胞分化而变化。

Immunogen

KLH缀合合成的2X支肽,包含序列..RKme2STG..,其中Kme2对应于人组蛋白H3残基9处的二甲基赖氨酸。
表位:H3K9me2

Application

免疫细胞化学分析:代表性批次的1:100稀释液可在HepG2细胞中检测到二甲基化组蛋白H3(Lys9)。
非偶联抗体(目录号07-441)也适用于蛋白质印迹法、斑点印迹法、免疫细胞化学和肽抑制试验(肽阻断)。
抗二甲基化组蛋白H3(Lys9)抗体,AlexaFluor 488偶联物是针对二甲基化组蛋白H3(Lys9)抗体的抗体,用于免疫细胞化学。
研究子类别
核受体
研究类别
表观遗传学&核功能

Biochem/physiol Actions

赖氨酸9二甲基化的组蛋白H3。

Physical form

蛋白A纯化
纯化的兔抗体偶联物溶于含15 mg/ml BSA和0.1 %叠氮化钠的PBS中。

Preparation Note

自收到之日起,在2-8°C条件下可稳定保存1年。

Analysis Note

通过免疫细胞化学在HeLa细胞中进行评估。

免疫细胞化学分析:该抗体的1:100稀释液在HeLa细胞中检测到二甲基化组蛋白H3(Lys9)。

Other Notes

浓度:请参考特定批次的数据表。

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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