Anti-trimethyl Histone H3 (Lys27), Alexa Fluor^™ 555 Conjugate Antibody

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified however Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Trimethylation of histone H3 on Lys9 (H3K9me3) is one of the most highly studied epigenetic marks. H3K9me3 functions in the repression of euchromatic genes, and in epigenetic control of heterochromatin assembly, most likely by acting as a recognition motif for the binding of chromatin-associated proteins, such as Swi6 or HP1α. The enzymes responsible for H3K9 trimethylation are SUV39H1 and SUV39H2.

~17 kDa observed. The unconjugated parent antibody (Cat. No. 07-449) has an observed molecular weight at ~17 kDa

Branch peptide corresponding to trimethyl-Histone H3 (Lys27).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

This Anti-trimethyl Histone H3 (Lys27) is validated for use in Immunocytochemistry for the detection of trimethyl Histone H3 (Lys27).

The unconjugated parent antibody of this antibody is Cat. No. 07-449.

Protein A purified

Purified rabbit IgG in PBS containing 15 mg/mL BSA and 0.1% Sodium Azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected trimethyl-Histone H3 (Lys27) in HeLa cells.
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Concentration: Please refer to lot specific datasheet.

ALEXA FLUOR is a trademark of Life Technologies

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