产品名称
Anti-B-Raf Antibody, Upstate®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... BRAF(673)
mouse ... Braf(109880)
rat ... Rasd1(64455)
Analysis Note
routinely evaluated by immunoblot in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme
Application
Research Category
Signaling
Signaling
Research Sub Category
MAP Kinases
MAP Kinases
This Anti-B-Raf Antibody is validated for use in IP, WB for the detection of B-Raf. This B-Raf antibody has been tested in Human, Mouse, and Rat.
Biochem/physiol Actions
full length recombinant BRaf (plus 6His tag) and B-Raf splice variants in cell lysates
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
94kDa
Immunogen
synthetic peptide corresponding to amino acids 754-765 of human B-Raf
Other Notes
Replaces: 04-328
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl,0.05% sodium azide before the addition of glycerol to 30%
Format: Purified
Protein A chromatography
Preparation Note
2 years at -20°C
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
Gang Ren et al.
International journal of cell biology, 2012, 913242-913242 (2012-05-23)
Recent high-throughput-sequencing of the cancer genome has identified oncogenic mutations in BRaf genetic locus as one of the critical events in melanomagenesis. In normal cells, the activity of BRaf is tightly regulated. Gain-of-function mutations like those identified in melanoma frequently
Sandeep S Joshi et al.
PloS one, 13(2), e0191264-e0191264 (2018-02-27)
Heat shock protein 90 (HSP90) is a molecular chaperone which stabilizes client proteins with important roles in tumor growth. 17-allylamino-17-demethoxygeldanamycin (17-AAG), an inhibitor of HSP90 ATPase activity, occupies the ATP binding site of HSP90 causing a conformational change which destabilizes
Lack of association between BRAF V600E mutation and mitogen-activated protein kinase activation in papillary thyroid carcinoma.
Hui Zuo,Yasushi Nakamura,Hironao Yasuoka,Ping Zhang,Misa Nakamura,Ichiro Mori et al.
Pathology International null
Regulation of B-Raf kinase activity by tuberin and Rheb is mammalian target of rapamycin (mTOR)-independent.
Karbowniczek, M; Cash, T; Cheung, M; Robertson, GP; Astrinidis, A; Henske, EP
The Journal of Biological Chemistry null
Kiril Bidzhekov et al.
Journal of cellular and molecular medicine, 11(6), 1395-1407 (2008-01-22)
Mouse embryonic endothelial progenitor cells (eEPCs) acquire a mature phenotype after treatment with cyclic adenosine monophosphate (cAMP), suggesting an involvement of Raf serine/threonine kinases in the differentiation process. To test this idea, we investigated the role of B-Raf and C-Raf
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