生物来源
rabbit
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
E. coli
制造商/商品名称
Upstate®
技术
western blot: suitable
同位素/亚型
IgG
运输
wet ice
靶向翻译后修饰
unmodified
一般描述
Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+-binding protein acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors. It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase.
The molecular weight is related to the tagged protein.
免疫原
KLH-conjugated, synthetic peptide (MKRRWKKNFIAVSAANRFKKISSSGAL) corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors. The immunizing sequence is identical in rabbit, mouse, and rat.
应用
Anti-Calmodulin Binding Protein Epitope Tag Antibody detects level of Calmodulin Binding Protein Epitope Tag & has been published & validated for use in WB.
生化/生理作用
Recognizes recombinant proteins containing the calmodulin binding protein epitope tag.
外形
Purified rabbit IgG in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide, pH7.4.
分析说明
Control
Lysates from transformed E. coli.
Lysates from transformed E. coli.
Routinely evaluated by Western Blot in lysates from transformed E. coli.
Western Blot Analysis:
A 1:2000-1:10000 dilution of this lot detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.
Western Blot Analysis:
A 1:2000-1:10000 dilution of this lot detected a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E. coli.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Stephen M T Hoke et al.
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Weijun Chen et al.
RNA (New York, N.Y.), 20(3), 308-320 (2014-01-21)
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Role of km23-1 in RhoA/actin-based cell migration.
Jin, Q; Pulipati, NR; Zhou, W; Staub, CM; Liotta, LA; Mulder, KM
Biochemical and biophysical research communications null
Ingo Bauer et al.
mBio, 7(6) (2016-11-03)
Histone deacetylases (HDACs) remove acetyl moieties from lysine residues at histone tails and nuclear regulatory proteins and thus significantly impact chromatin remodeling and transcriptional regulation in eukaryotes. In recent years, HDACs of filamentous fungi were found to be decisive regulators
Development of the "Three-step MACS": a novel strategy for isolating rare cell populations in the absence of known cell surface markers from complex animal tissue.
Lee, MY; Lufkin, T
Journal of biomolecular techniques : JBT null
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