Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors including p47phox, p67phox, p40phox and Rac (1 or 2). In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight complex which can be recovered from the cytosolic fraction. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane. p47phox, p67phox, and Rac exhibit cooperative binding, since increasing the concentration of any one component lowers the EC50 of the other components. A great deal of current research involves understanding the protein protein interactions, and how these change with the activation state.

~47 kDa

KLH-conjugated, synthetic peptides containing the first 15 amino acids of the N-terminal and the last 15 amino acids of the C-terminus of mouse p47-phox.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

This Anti-p47-phox (mouse) Antibody is validated for use in WB for the detection of p47-phox (mouse).

Recognizes mouse p47-phox, MW ~47 kDa.

Protein A purified

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

Stable for 1 year at -20ºC from date of receipt.

Control
RAW 264.7 cell lysate.

Evaluated by western blot in RIPA lysates from RAW 264.7 cells.

Western Blot Analysis:
0.5-2.0 µg/mL of this antibody detected p47-phox in RIPA lysates from RAW 264.7 cells.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-240

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