07-687
抗细胞周期蛋白E抗体
Upstate®, from rabbit
别名:
Anti-CCNE, Anti-pCCNE1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IHC, IP, WB
Citations:
34
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CCNE1(898)
General description
Mr 52kDa
细胞周期蛋白 E 是 Cdk2 的调节亚基，在哺乳动物细胞周期中控制 G1/S 的转变。细胞周期蛋白 E 的多种同工型仅在肿瘤中表达，而在正常组织中不表达，这表明细胞周期蛋白 E 受转录后调控。体外分析表明，细胞周期蛋白 E 的这些截短的变体能够磷酸化组蛋白 H1。细胞周期蛋白 E 异常被认为是各种癌症预后较差的指标。
Immunogen
KLH偶联合成肽，对应于大鼠细胞周期蛋白E的氨基酸378-396（GVLTPPHSSKKQSSEQETE）。
Application
使用经验证可用于IP、WB & IHC的抗细胞周期蛋白E抗体（兔多克隆抗体）检测细胞周期蛋白E。
研究子类别
细胞周期，DNA 复制&修复
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
细胞周期蛋白E1
Physical form
免疫亲和纯化
含有0.1%叠氮化钠和0.2%明胶的PBS 。
Preparation Note
自发货之日起在4°C可稳定保存1年。
Analysis Note
对照
阳性抗原对照：货号12-305，3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液，煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
阳性抗原对照：货号12-305，3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液，煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
已通过免疫印迹法对3T3/A31细胞的RIPA裂解液进行了常规评估
Other Notes
替代：04-223
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Cyclin A: function and expression during cell proliferation.
Desdouets, C, et al.
Progress in Cell Cycle Research, 1, 115-123 (1995)
Philipp Schuler et al.
Plant, cell & environment, 45(1), 12-22 (2021-09-27)
The analysis of the non-exchangeable hydrogen isotope ratio (δ2 Hne ) in carbohydrates is mostly limited to the structural component cellulose, while simple high-throughput methods for δ2 Hne values of non-structural carbohydrates (NSC) such as sugar and starch do not
Xiaoyun Tang et al.
Theranostics, 12(13), 5675-5690 (2022-08-16)
LPP2 is one of three enzymes in the lipid phosphate phosphatase family (LPP1-3) that dephosphorylate extracellular and intracellular bioactive lipid phosphates and pyrophosphates. LPP2 increases cell growth and LPP2 expression is elevated in a variety of malignancies, implying that LPP2
The role of cyclin E in the regulation of entry into S phase.
Sauer, K and Lehner, C F
Progress in Cell Cycle Research, 1, 125-139 (1995)
Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2.
Prall, O W, et al.
The Journal of Biological Chemistry, 272, 10882-10894 (1997)
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